Differential induction from X-irradiated human peripheral blood monocytes to dendritic cells
Differential induction from X-irradiated human peripheral blood monocytes to dendritic cells
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DOI：
10.1269/jrr.07122
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发表时间：
2008-05-01
影响因子：
2
通讯作者：
Kashiwakura, Ikuo
中科院分区：
文献类型：
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作者：
Yoshino, Hironori;Takahashi, Kenji;Kashiwakura, Ikuo
树突状细胞（Dendritic cells，DCs）是一类在免疫系统中起重要作用的抗原提呈细胞。为了阐明电离辐射对分化为DC的影响，我们集中于人外周血单核细胞，并研究X射线照射的单核细胞是否可以分化为DC。用适当的细胞因子刺激将未照射的单核细胞和5Gy照射的单核细胞诱导成未成熟DC（iDC）和成熟DC（mDC），并且来自每个单核细胞的诱导细胞表达每个DC表达表面抗原，例如CD 40、CD 86和HLA-DR。来源于5Gy照射的单核细胞的iDC上的CD 40和CD 86的表达水平高于来源于未照射的单核细胞的iDC。此外，与来自未照射的单核细胞的mDC相比，来自5 Gy照射的单核细胞的mDC具有显著更低的刺激同种异体T细胞的能力。未照射和照射的单核细胞的DC调节的上清液中检测到的iDC和细胞因子的吞噬活性没有显著差异。这些结果表明，暴露于电离辐射的人单核细胞可以因此分化为DC，但是存在X射线照射导致DC功能受损的趋势。
Dendritic cells (DCs) are a type of antigen-presenting cell which plays an essential role in the immune system. To clarify the influences of ionizing radiation on the differentiation to DCs, we focused on human peripheral blood monocytes and investigated whether X-irradiated monocytes can differentiate into DCs. The non-irradiated monocytes and 5 Gy-irradiated monocytes were induced into immature DCs (iDCs) and mature DCs (mDCs) with appropriate cytokine stimulation, and the induced cells from each monocyte expressed each DC-expressing surface antigen such as CD40, CD86 and HLA-DR. However, the expression levels of CD40 and CD86 on the iDCs derived from the 5 Gy-irradiated monocytes were higher than those of iDCs derived from non-irradiated monocytes. Furthermore, the mDCs derived from 5 Gy-irradiated monocytes had significantly less ability to stimulate allogeneic T cells in comparison to the mDCs derived from non-irradiated monocytes. There were no significant differences in the phagocytotic activity of the iDCs and cytokines detected in the supernatants conditioned by the DCs from the non-irradiated and irradiated monocytes. These results suggest that human monocytes which are exposed to ionizing radiation can thus differentiate into DCs, but there is a tendency that X-irradiation leads to an impairment of the function of DCs.