Strong Electrochemiluminescence from MOF Accelerator Enriched Quantum Dots for Enhanced Sensing of Trace cTnI
Strong Electrochemiluminescence from MOF Accelerator Enriched Quantum Dots for Enhanced Sensing of Trace cTnI
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MOF 加速器富集量子点产生的强电化学发光可增强痕量 cTnI 的传感
DOI：
10.1021/acs.analchem.7b05137
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发表时间：
2018-03-20
影响因子：
7.4
通讯作者：
Zhuo, Ying
中科院分区：
文献类型：
--
作者：
Yang, Xia;Yu, Yan-Qing;Zhuo, Ying
电化学发光（ECL）生物检测技术的发展依赖于信号强度高且稳定的ECL信号标签。在这项工作中，从金属有机框架（MOF）加速器富集的量子点（CdTe），这是一个有效的ECL信号标签，痕量生物标志物检测实现了强的ECL发射。特别值得注意的是，建立了一种新的机制，大大提高了CdTe的电化学发光强度，因为等网格金属有机框架-3（IRMOF-3）与2-氨基对苯二甲酸（2-NH 2-BDC）作为有机配体不仅允许通过包封效应和内部/外部装饰，但也作为一种新的共反应物促进剂，用于促进共反应物S2 O 82-转化为硫酸根阴离子（SO 4中心点-），进一步提高CdTe的ECL发射。在简单的夹心免疫反应方法的基础上，选择心肌梗死相关生物标志物心肌肌钙蛋白I抗原（cTnI）作为检测模型，使用富含IRMOF-3的CdTe标记抗体作为信号探针。该免疫传感器表现出理想的cTnI检测性能，响应范围从1.1 fg mL（-1）至11 ng mL（-1），检测限非常低（0.46 fg mL（-1））。这表明IRMOF-3富集的CdTe纳米复合材料策略可以将共反应物加速剂和发光体结合起来，显著提高ECL的强度和稳定性，为具有广泛应用前景的ECL标签的制备提供了方向。
The development of a sensitive and practical electrochemiluminescence (ECL) bioassay relies on the use of ECL signal tags whose signal intensity is high and stable. In this work, strong ECL emission was achieved from metal organic framework (MOF) accelerator enriched quantum dots (CdTe), which were applied as an efficient ECL signal tag for trace biomarker detection. It is particularly noteworthy that a novel mechanism to drastically enhance the ECL intensity of CdTe is established because isoreticular metal organic framework-3 (IRMOF-3) with 2-amino terephthalic acid (2-NH2-BDC) as the organic ligand not only allows for loading a large amount of CdTe via the encapsulating effect and internal/external decoration but also functions as a novel coreactant accelerator for promoting the conversion of coreactant S2O82- into the sulfate radical anion (SO4 center dot-), further boosting the ECL emission of CdTe. On the basis of the simple sandwich immunoreaction approach, cardiac troponin-I antigen (cTnI), a kind of biomarker related with myocardial infarction, was chosen as a detection model using an IRMOF-3-enriched CdTe labeled antibody as the signal probe. This immunosensor demonstrated desirable assay performance for cTnI with a wide response range from 1.1 fg mL(-1) to 11 ng mL(-1) and a very low detection limit (0.46 fg mL(-1)). This suggested that the IRMOF-3-enriched CdTe nanocomposite strategy can integrate the coreactant accelerator and luminophore to significantly enhance the ECL intensity and stability, providing a direction for promising ECL tag preparation with broad applications.