Sequencing and analysis of the complete organellar genomes of Parmales, a closely related group to Bacillariophyta (diatoms)
Sequencing and analysis of the complete organellar genomes of Parmales, a closely related group to Bacillariophyta (diatoms)
复制标题
DOI：
10.1007/s00294-016-0598-y
复制
发表时间：
2016-11-01
期刊：
影响因子：
2.5
通讯作者：
Sato, Naoki
中科院分区：
文献类型：
--
作者：
Tajima, Naoyuki;Saitoh, Kenji;Sato, Naoki
我们测定了单细胞海洋浮游植物Triparma laevis的完整的叶绿体和线粒体基因组，属于Parmales(异形目)。Parmales的细胞被硅化的细胞壁包围，类似于硅藻(硅藻)。根据SSU、rDNA和rbcL序列进行的分子系统发育分析表明，莱维氏锥藻与硅藻为姊妹群。硅藻是现代海洋中最成功的浮游植物类群，但其起源和早期演化尚不清楚。对莱维氏毛藻进行详细的分子分析可能会增加我们对巴马目和硅藻之间进化关系的理解。莱维氏毛藻和硅藻的叶绿体和线粒体基因组的基因含量相似。叶绿体基因组的基因序列也与硅藻相似，而线粒体基因组的基因序列在硅藻中并不保守，但结构比硅藻更紧凑。利用叶绿体编码的串联氨基酸数据集和线粒体编码的串联氨基酸数据集进行的系统发育分析表明，莱维氏毛藻是硅藻的姊妹群。这些结果表明，莱维氏毛藻的细胞器基因组特征与硅藻相似，并比硅藻更能保留祖先特征。
We sequenced the complete plastid and mitochondrial genomes of the unicellular marine phytoplankton Triparma laevis, belonging to the order Parmales (Heterokonta). The cells of Parmales are surrounded by silicified cell walls, similar to Bacillariophyta (diatoms). T. laevis was recognized as a sister group of Bacillariophyta using a molecular phylogenetic analysis based on SSU rDNA and rbcL sequences. Bacillariophyta are the most successful group of phytoplankton in the modern ocean, but the origin and early evolution of them have not been clearly established. Detailed molecular analyses of T. laevis may increase our understanding of the evolutionary relationships among Parmales and Bacillariophyta. The gene contents of the plastid and mitochondrial genomes are similar between T. laevis and Bacillariophyta. The gene order of the plastid genome is also similar to Bacillariophyta, whereas the gene order of the mitochondrial genome is not conserved in Bacillariophyta, but the structure is more compact than Bacillariophyta. Phylogenetic analyses, using plastid-encoded concatenated amino acid datasets and mitochondria-encoded concatenated amino acid datasets suggest that T. laevis is a sister group of Bacillariophyta. These results suggest that the characteristics of the organellar genomes of T. laevis are similar and conserve ancestral characteristics more than Bacillariophyta.