Postnatal development of 2 microcircuits involving fast-spiking interneurons in the mouse prefrontal cortex.
Postnatal development of 2 microcircuits involving fast-spiking interneurons in the mouse prefrontal cortex.
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DOI：
10.1093/cercor/bhs291
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Jian-Ming Yang;Jing Zhang;Yan-Qin Yu;S. Duan;Xiao-Ming Li
中科院分区：
文献类型：
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作者：
Jian-Ming Yang;Jing Zhang;Yan-Qin Yu;S. Duan;Xiao-Ming Li
前额叶皮质(PFC)小白蛋白阳性快脉冲(FS)中间神经元发育异常与精神分裂症、自闭症等多种神经精神障碍密切相关。在PFC内，FS中间神经元至少形成两个微回路：一个通过化学突触与锥体神经元(FS-PN)相连，另一个通过化学突触和电突触与其他FS中间神经元(FS-FS)相连。目前尚不清楚这些电路是何时以及如何在早期开发的PFC中建立的。在这里，我们使用G42小鼠，用增强的绿色荧光蛋白特异性地标记FS中间神经元，从出生后第3天(P3)到P30进行双细胞记录，以研究Fs在PFC中的神经元间网络的发育。我们发现，在出生后1周，FS中间神经元的膜和网络特性发育不良，在出生后2周，两者都呈现出突然成熟的趋势。FS神经元间微路的发育贯穿整个成年期早期。因此，我们的数据表明，在出生后第一周，FS中间神经元可能不参与皮质振荡活动和γ振荡的产生。我们的数据还表明，在早期，FS神经元间网络之间的电和化学突触独立发展。
Disturbed development of the parvalbumin-positive fast-spiking (FS) interneurons in the prefrontal cortex (PFC) is closely associated with many neuropsychiatric disorders such as schizophrenia and autism. FS interneurons form at least 2 microcircuits in the PFC: one with pyramidal neurons (FS-PN) through chemical synapses; the other with other FS interneurons (FS-FS) via chemical and electrical synapses. It is currently unknown when and how these circuits are established in the PFC during early development. Here, we used G42 mice, in which FS interneurons are specifically labeled with enhanced green fluorescent protein, to make dual whole-cell recordings from postnatal day 3 (P3) to P30 to study the development of FS interneuronal networks in the PFC. We found that FS interneurons were poorly developed in terms of the membrane and network properties during the first postnatal week, both of which exhibited an abrupt maturation during the second postnatal week. The development of FS interneuronal microcircuits lasted throughout early adulthood. Thus, our data suggest that FS interneurons might not be involved in generating cortical oscillatory activity and γ oscillations during the first postnatal week. Our data also indicate an independent development of electrical and chemical synapses among FS interneuronal networks during the early period.