Large scale analysis of virulence genes in Escherichia coli strains isolated from Avalon Bay, CA
Large scale analysis of virulence genes in Escherichia coli strains isolated from Avalon Bay, CA
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DOI：
10.1016/j.watres.2010.06.058
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发表时间：
2010-10-01
期刊：
影响因子：
12.8
通讯作者：
Sadowsky, Michael J.
中科院分区：
文献类型：
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作者：
Hamilton, Matthew J.;Hadi, Asbah Z.;Sadowsky, Michael J.
娱乐沃茨被大肠杆菌和肠球菌污染是一个普遍的问题，导致海滩关闭和娱乐活动的损失。而E.大肠杆菌经常被用作粪便污染的指标，并已在水道中广泛测量，很少有研究检查潜在致病性大肠杆菌的存在。海滩沃茨中的大肠杆菌菌株。在这项研究中，结合高通量，机器人辅助的菌落杂交和PCR分析，以确定基因组组成和存在于大肠杆菌中的毒力基因的频率。大肠杆菌分离自加利福尼亚州圣卡塔利纳岛阿瓦隆湾的海滩水。共24,493人。2007年8月至9月和2008年7月至8月，在一个受欢迎的游泳海滩的两个地点收集了大肠杆菌分离株。检测所有分离株是否存在志贺样毒素（stx 1/stx 2）、粘附素（eaeA）和肠毒素（ST/LT）。在检测的24，493株分离株中，3.6%含有eaeA基因，表明这些分离株是潜在的EPEC菌株。然而，在五个日期，超过10%的菌株是潜在的EPEC，这表明在这个海滩的毒力基因的发病率有很强的时间组成部分。没有检测到STEC或ETEC分离株，只有8个（
Contamination of recreational waters with Escherichia coli and Enterococcus sp. is a widespread problem resulting in beach closures and loss of recreational activity. While E. coli is frequently used as an indicator of fecal contamination, and has been extensively measured in waterways, few studies have examined the presence of potentially pathogenic E. coli strains in beach waters. In this study, a combination of high-throughput, robot-assisted colony hybridization and PCR-based analyses were used to determine the genomic composition and frequency of virulence genes present in E. coli isolated from beach water in Avalon Bay, Santa Catalina Island, CA. A total of 24,493 E. coli isolates were collected from two sites at a popular swimming beach between August through September 2007 and from July through August 2008. All isolates were examined for the presence of shiga-like toxins (stx1/stx2), intimin (eaeA), and enterotoxins (ST/LT). Of the 24,493 isolates examined, 3.6% contained the eaeA gene, indicating that these isolates were potential EPEC strains. On five dates, however, greater than 10% of the strains were potential EPEC, suggesting that incidence of virulence genes at this beach has a strong temporal component. No STEC or ETEC isolates were detected, and only eight (