Importance of non-systemic leaf autophagy for suppression of zinc starvation induced-chlorosis
Importance of non-systemic leaf autophagy for suppression of zinc starvation induced-chlorosis
复制标题
非系统性叶片自噬对于抑制锌饥饿引起的失绿症的重要性
DOI：
10.1080/15592324.2020.1746042
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Shinozaki;D.;Notaguchi;M.;and Yoshimoto;K.
自噬是一种自降解系统，在缺锌（−Zn）的植物中促进细胞内锌（Zn）的循环。因此，自噬缺陷的植物在-Zn下表现出严重的失绿现象。 根是直接暴露于缺锌环境的植物器官，然而，在我们最近的研究中，在叶子中明显观察到-Zn症状，表现为失绿症。在这里，我们进行了微移植，以确定哪个器官的自噬活性对于抑制 -Zn 诱导的萎黄症很重要。仅在根或叶中具有自噬活性的嫁接植物在 -Zn 下生长，然后比较它们之间的褪绿表型。因此，无论砧木自噬活性如何，只有当接穗自噬有缺陷时，-Zn 诱导的叶片失绿才会发生。该数据表明，通过根细胞自噬降解重新供应的锌不能有助于抑制叶片失绿。因此，地上部分的自噬对于控制-Zn诱导的叶片失绿症至关重要。结合我们最近报道的数据，我们得出结论，通过自噬降解补充锌的机制不是整个植物的系统性，而是局部系统。
Autophagy, which is one of the self-degradation systems, promotes intracellular zinc (Zn) recycling under Zn deficiency (−Zn) in plants. Therefore, autophagy defective plants show severe chlorosis under −Zn. Root is the plant organ which directly exposed to Zn deficient environment, however, in our recent study, −Zn symptom was prominently observed in leaves as chlorosis. Here, we conducted micrografting to determine which organ’s autophagic activities are important to suppress the −Zn induced chlorosis. Grafted plants that have autophagic activities only in roots or leaves were grown under −Zn and then compared chlorotic phenotypes among them. As a result, regardless of the autophagic activities in rootstocks, −Zn induced-chlorosis in leaves was occurred only when autophagy in scion was defective. This data indicates that Zn resupplied by autophagic degradation in root cells could not contribute to suppress the chlorosis in leaves. Thus, autophagy in the aerial part is critical for controlling −Zn induced-chlorosis in leaves. Taken together, along with our recently reported data, we conclude that the mechanism of Zn resupply by autophagic degradation is not systemic throughout the plant but rather a local system.
影响因子：
7.4
作者：
Hanaoka, H;Noda, T;Ohsumi, Y
通讯作者：
Ohsumi, Y
影响因子：
11.6
作者：
Yoshimoto, Kohki;Jikumaru, Yusuke;Shirasu, Ken
通讯作者：
Shirasu, Ken