COMPLEX PATTERN OF THE MYELOMONOCYTIC DIFFERENTIATION ANTIGENS MRP8 AND MRP14 DURING CHRONIC AIRWAY INFLAMMATION
COMPLEX PATTERN OF THE MYELOMONOCYTIC DIFFERENTIATION ANTIGENS MRP8 AND MRP14 DURING CHRONIC AIRWAY INFLAMMATION
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DOI：
10.1016/s0171-2985(11)80259-7
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发表时间：
1992-11-01
期刊：
影响因子：
2.8
通讯作者：
SORG, C
中科院分区：
文献类型：
--
作者：
ROTH, J;TEIGELKAMP, S;SORG, C
囊性纤维化的特征之一是患者血浆中存在所谓的囊性纤维化抗原。CF-抗原已显示由两种钙结合蛋白MRP 8和MRP 14组成。在本研究中，我们首先研究了血浆中MRP 8和MRP 14滴度的升高是否与CF的原发性缺陷有关，或者是慢性气道炎症的结果;第二，通过采用ELISA技术，我们测量了患有CF或非特异性慢性支气管炎（我们发现，与健康对照组相比，CF和CB患者的唾液中这两种蛋白质的血浆浓度升高。水平与疾病活动的全身和局部体征（即C反应蛋白（CRP）、每日痰液生成量）显著相关。MRP 8和MRP 14在CF和CB患者的痰液中以相似的浓度被发现，并且在CF患者和健康供体的唾液中以较小的程度被发现。经共价交联后，在所有样品中检测到至少三种不同的由MRP 8和MRP 14组成的复合物，其分子量约为25、35和48 kDa，由此我们得出结论，CF和CB中MRP 8和MRP 14的血浆水平升高是炎症过程的结果。此外，这些蛋白质的可能生物学功能似乎与MRP 8和MRP 14的复合形式有关，而不是与单个蛋白质有关。
One of the characteristics of cystic fibrosis is the presence of the so-called cystic fibrosis antigen in the plasma of patients. The CF-antigen has been shown to consist of the two calcium-binding proteins MRP8 and MRP14. In the present study we investigate first whether elevated plasma titers of MRP8 and MRP14 are linked to the primary defect of CF or are rather a result of chronic airway inflammation; and second, whether the known complexes of these proteins may have in vivo relevance during inflammation.By employing the ELISA technique we measured MRP8 and MRP14 levels in the plasma of patients suffering from CF or nonspecific chronic bronchitis (CB) and of healthy controls, in sputum of CF and CB patients, and in saliva of CF patients and healthy controls, respectively.We found elevated plasma concentrations of both proteins in CF and CB patients compared to healthy controls. Levels correlated significantly with systemic and local signs of disease activity (i.e. c-reactive protein (CRP), daily sputum production). MRP8 and MRP14 both were found in high amounts at similar concentrations in sputum of CF and CB patients and, to a lesser extent, in saliva of CF patients and healthy donors. After covalent cross-linking at least three different complexes composed of MRP8 and MRP14 with approximate molecular weights of about 25, 35 and 48 kDa were detected in all samples.From this we conclude that the elevated plasma levels of MRP8 and MRP14 in CF and CB are the result of inflammatory processes. Further, possible biological functions of these proteins seem to be associated with complexed forms of MRP8 and MRP14 rather than with individual proteins.