3-DIMENSIONAL STRUCTURE OF ASTROCYTES IN THE RAT DENTATE GYRUS
3-DIMENSIONAL STRUCTURE OF ASTROCYTES IN THE RAT DENTATE GYRUS
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DOI：
10.1002/cne.902490209
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发表时间：
1986-07-08
影响因子：
2.5
通讯作者：
HAMA, K
中科院分区：
文献类型：
--
作者：
KOSAKA, T;HAMA, K
本研究采用高尔基体浸渍材料光镜和高压电镜、常规电镜、高尔基体-电镜联合法和胶质纤维酸性蛋白免疫组化等方法，对大鼠齿状回星形胶质细胞的结构特征进行了研究。齿状回中的星形胶质细胞为原生质型，并根据其胞体的位置分为六种亚型：即，星形胶质细胞（1）在多形层中，（2）在颗粒下区中，（3）在颗粒细胞层中，（4）在颗粒细胞层和分子层的边界处，（5）在分子层中，和（6）在软脑膜表面下。高尔基体浸渍材料的5 μ m厚切片的立体观察揭示了星形胶质细胞过程的三维结构细节，其在光学显微镜或常规的薄片电子显微镜中不明显。它们大多数基本上都是薄片，形状和大小根据它们的位置而变化。在颗粒细胞层中，薄的面纱状片或薄片，起源于三种星形胶质细胞（亚型2，3和4），干预颗粒细胞胞体之间，而在丛状和分子层小叶状附属物起源于星形胶质细胞（亚型1，2，4和5）相互混合，使海绵状团块。因此，在颗粒细胞层和分子层的边界的颗粒下区的星形胶质细胞表现出显着的区域分化，其层间的过程。除了这些片状工艺之外，细线状工艺也很常见。
Structural features of astrocytes in the rat dentate gyrus were studied by means of light and high-voltage electron microscopy of Golgi-impregnated materials, conventional electron microscopy, combined Golgi-electron microscopy, and immunohistochemistry for glial fibrillar acidic protein. Astrocytes in the dentate gyrus were of the protoplasmic type and were classified into six subtypes based on the location of their somata: i.e., astrocytes (1) in the polymorph layer, (2) in the subgranular zone, (3) in the granular cell layer, (4) at the border of the granular cell layer and the molecular layer, (5) in the molecular layer, and (6) subjacent to the pia surface. Stereoscopic observations of 5-.mu.m-thick sections of Golgi-impregnated materials revealed three-dimensional structural details of astroglial processes not apparent in either the light microscope or in conventional thin-section electron microscopy. Most of them were basically thin sheets, varying in shape and size in accordance with their sites. In the granule cell layer, thin veil-like sheets or lamellae, originating from three kinds of astrocytes (subtypes 2, 3, and 4), intervened between granule cell somata, whereas in the plexiform and molecular layers small leaflet-like appendages originating from astrocytes (subtypes 1, 2, 4, and 5) intermingled with one another, making sponge-like conglomerates. Thus astrocytes in the subgranular zone and those at the border of the granule cell layer and the molecular layer showed prominent regional differentiation of their processes among layers. In addition to these sheet-like processes, thin thread-like processes were also common.