A saponin correlated with variable resistance of Barbarea vulgaris to the diamondback moth Plutella xylostella
A saponin correlated with variable resistance of Barbarea vulgaris to the diamondback moth Plutella xylostella
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DOI：
10.1023/a:1024217504445
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发表时间：
2003-06-01
影响因子：
2.3
通讯作者：
Renwick, JAA
中科院分区：
文献类型：
--
作者：
Agerbirk, N;Olsen, CE;Renwick, JAA
两种类型的普通藤本植物。G型和P型对小菜蛾幼虫的抗性不同。G型莲座状植物在温室中生长或在夏季采集时对DBM具有完全抗性，但在晚秋采集的叶片对跳甲的抗性较弱，正如之前发现的对跳甲的抗性。P型总是易感的。在生物测定中，抗性小叶的提取物抑制幼虫的生长，并通过活性引导分级分离得到一个生长抑制组分。从该部位分离得到一个三萜皂苷(1)，经光谱分析和水解物分析鉴定为3-O-β-纤维二糖齐墩果酸。G型阻力的下降与1水平的下降相关，从0.6-0.9下降到
Two types of Barbarea vulgaris var. arcuata, the G-type and the P-type, differed in resistance to larvae of the diamondback moth (DBM) Plutella xylostella. Rosette plants of the G-type were fully resistant to the DBM when grown in a greenhouse or collected in the summer season, but leaves collected during the late fall were less resistant, as previously found for flea beetle resistance. The P-type was always susceptible. Extracts of resistant leaflets inhibited larval growth in a bioassay, and a growth-inhibiting fraction was isolated by activity-guided fractionation. A triterpenoid saponin (1) was isolated from this fraction and identified as 3-O-beta-cellobiosyloleanolic acid from spectroscopic data and analysis of hydrolysis products. The decrease in resistance of the G-type in the fall was correlated with a decrease in the level of 1, from 0.6-0.9 to