COMPARISON OF THE INVITRO TRANSLATION PRODUCTS OF WILD-TYPE AND A DELETION MUTANT OF SOIL-BORNE WHEAT MOSAIC-VIRUS
COMPARISON OF THE INVITRO TRANSLATION PRODUCTS OF WILD-TYPE AND A DELETION MUTANT OF SOIL-BORNE WHEAT MOSAIC-VIRUS
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DOI：
10.1099/0022-1317-67-7-1237
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发表时间：
1986-07-01
影响因子：
3.8
通讯作者：
EHARA, Y
中科院分区：
文献类型：
--
作者：
SHIRAKO, Y;EHARA, Y
将土传小麦花叶病毒（SBWMV）S野生型分离物（WT）（RNA Ⅰ 6.9kb，RNA Ⅱ 3.6kb）通过机械接种法连续转移到小麦植株中，获得了RNA Ⅰ 6.9kb，RNA Ⅱ 2.1kb的缺失突变体（DM）。在小麦胚芽提取物中，WT和DM的RNA I指导具有mol.重量220000（220K）和150K的RNA II，以及WT和DM的RNA II指导25K和19K多肽的合成。在用碱性缓冲液纯化的DM病毒体的WT的小麦胚芽提取物中孵育也得到作为主要产物的220K、25K和19D多肽和作为次要产物的150K多肽。在兔网织红细胞裂解物中。WT和DM的RNA I仅指导220K多肽的合成，而WT RNA II产生100K、46K、25K和19K多肽，DM RNA II产生31K、25K和19K多肽。SBWMV DM抗血清沉淀100K、31K、25K和19K多肽，但不沉淀220K和46K多肽。根据19K多肽的电泳迁移率及其与病毒颗粒的抗血清的反应，鉴定其为衣壳蛋白。因此，WT和DM SBWMV的体外翻译产物之间的差异仅限于由RNA II编码的那些。
S wild-type isolate (WT) of soil-borne wheat mosaic virus (SBWMV, isolate JT) (RNA I 6.9 kb and RNA II 3.6 kb) was successively transferred to wheat plants by mechanical inoculation and a deletion mutant (DM) (RNA I 6.9 kb and RNA II 2.1 kb) was produced. In wheat germ extracts, RNA I of both WT and DM directed synthesis of polypeptides having mol. wt. of 220000 (220K) and 150K, and RNA II of both WT and DM directed synthesis of 25K and 19K polypeptides. Incubation in wheat germ extracts of WT of DM virions purified with an alkaline buffer also gave 220K, 25K and 19D polypeptides as major products and a 150K polypeptide as a minor product. In rabbit reticulocyte lysates. RNA I of both WT and DM directed synthesis of only the 220K polypeptide, whereas WT RNA II produced 100K, 46K, 25K and 19K polypeptides and DM RNA II, 31K, 25K and 19K polypeptides. SBWMV DM antiserum precipitated polypeptides of 100K, 31K, 25K and 19K but not 220K and 46K. The 19K polypeptide was identified as the capsid protein from its electrophoretic mobility and its reaction with antiserum to virus particles. Thus, the differences between the in vitro translation products of WT and DM SBWMV were confined only to those coded by RNA II.