Determination of Dufulin Residue in Vegetables, Rice, and Tobacco Using Liquid Chromatography with Tandem Mass Spectrometry
Determination of Dufulin Residue in Vegetables, Rice, and Tobacco Using Liquid Chromatography with Tandem Mass Spectrometry
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使用液相色谱串联质谱法测定蔬菜、大米和烟草中的杜芙灵残留
DOI：
10.5740/jaoacint.15-134
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发表时间：
2015-11-01
影响因子：
1.6
通讯作者：
Zhang, Kankan
中科院分区：
文献类型：
--
作者：
Li, Yanjiao;Lu, Ping;Zhang, Kankan
建立了一种快速、准确的LC/MS/MS正离子电喷雾电离法测定烟叶（干）、番茄、黄瓜和大米样品中新型植物抗病毒药毒氟磷的残留量。样品用乙腈提取;通过使用伯仲胺、C-18和石墨化炭黑吸附剂的分散SPE净化;在C-18柱上分离;并通过多反应监测模式MS确认，基质效应为-21.5-19.6%。方法线性良好（R-2 >= 0.9912）。LOD和LOQ分别为0.05和0.15 μ g/kg。4种基质的平均回收率为71.9 ~ 93.6%，日内RSD为2.9 ~ 9.0%，日间RSD为6.9 ~ 15.2%。该方法已成功地应用于实际样品中毒福林的分析。
A rapid and accurate LC/MS/MS method using positive electrospray ionization was established for the determination of residues of the novel plant antiviral agent dufulin in samples of tobacco leaf (dry), tomato, cucumber, and rice. Samples were extracted with acetonitrile; cleaned up by dispersive SPE using primary secondary amine, C-18, and graphitized carbon black sorbents; separated on a C-18 column; and confirmed by multiple reaction monitoring mode MS with a matrix effect of -21.5-19.6%. The method showed satisfactory linearity (R-2 >= 0.9912) for the target compound. The LOD and the LOQ were 0.05 and 0.15 mu g/kg, respectively. The mean recoveries from four matrixes varied from 71.9 to 93.6% with intraday RSD in the range of 2.9 to 9.0% and interday RSD 6.9 to 15.2%. The method was successfully applied for analysis of dufulin in actual trial samples.