TISSUE-CULTURE INVESTIGATIONS OF BAMBOO .4. ORGANOGENESIS LEADING TO ADVENTITIOUS SHOOTS AND PLANTS IN EXCISED SHOOT APICES
TISSUE-CULTURE INVESTIGATIONS OF BAMBOO .4. ORGANOGENESIS LEADING TO ADVENTITIOUS SHOOTS AND PLANTS IN EXCISED SHOOT APICES
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DOI：
10.1016/0098-8472(89)90004-x
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发表时间：
1989-07-01
影响因子：
5.7
通讯作者：
CHEN, WL
中科院分区：
文献类型：
--
作者：
HUANG, LC;HUANG, BL;CHEN, WL
茎尖在2,4- d培养基中培养成乳白色的愈伤组织，继代培养的愈伤组织对生长素/细胞分裂素水平的变化不产生分化。相反，NAA和BA组合培养的愈伤组织为绿色、部分组织、粒状或结节状。愈伤组织的进一步培养保持了其部分组织状态，并产生了不定芽。新芽生根了，很容易移植到土壤中。器官源愈伤组织是由腋芽内的细胞强烈分裂而形成的，导致芽不规则膨胀，最终破裂。新梢原基在肿胀组织的浅层细胞中分化，特别是在有表皮的区域。表皮是原基的一部分。较老的培养主要表现为无定形愈伤组织的增加，通过在新形成的芽中发生肿胀，以及多芽的分化。颗粒状或结节状的组织结构是由于硬愈伤组织的聚集在发育的各个阶段穿插着芽。芽虽然是不定的，但不是由单个细胞产生的。
Shoot apices cultured in 2,4-D medium developed a creamy-white callus, subcultures of which do not differentiate shoots in response to variations of auxin/cytokinin levels. In contrast, those cultured in combinations of NAA and BA produced a green, partially organized, granular or nodular callus. Further culture of this callus maintained its partially organized state and resulted in the emergence of of adventitious shoots. The new shoots were rooted and readily transplanted in soil. The organogenic callus, developed by intense cell division within axillary buds, resulted in irregular swelling and eventual bursting of buds. New shoot primordia differentiated in superficial cell layers of swollen tissues and specifically in regions with an epidermis. The epidermis formed part of the primordium. Older cultures displayed principally an increase of amorphous callus through swelling occurring in newly formed buds, as well as differentiation of super-numerary buds. The granular or nodular texture was due to aggregates of hard callus interspersed with buds in all stages of development. Although adventitious, the buds were not derived from single cells.