Isolation of flagellated membrane vesicles from Caulobacter crescentus cells: evidence for functional differentiation of polar membrane domains.
Isolation of flagellated membrane vesicles from Caulobacter crescentus cells: evidence for functional differentiation of polar membrane domains.
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从新月柄杆菌细胞中分离有鞭毛的膜囊泡：极性膜域功能分化的证据。
DOI：
10.1073/pnas.81.11.3409
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发表时间：
1984
影响因子：
11.1
通讯作者：
Newton,A
中科院分区：
文献类型：
--
作者：
Huguenel,E;Newton,A
描述了免疫亲和层析程序，用于将新月柄杆菌细胞的膜囊泡分离成有鞭毛（极性）囊泡和无鞭毛（非极性）囊泡。二维凝胶电泳分析表明，许多蛋白质主要与极性或非极性部分相关，这一结果表明这些细胞的包膜组织成至少两个相对稳定的结构域。放射免疫测定还表明，鞭毛蛋白的膜池（已知其在鞭毛丝组装中充当前体）可能仅位于极性膜域中。因此，结果为 C. crescentus 细胞包膜的结构和功能分化提供了生化证据。这些发现与我们之前提出的模型一致，该模型用于解释表面结构对新月形细胞极的靶向。这里描述的免疫吸附方法应该有助于进一步研究这个问题，以及在其他系统中分离具有特征表面抗原的膜域。
An immunoaffinity chromatography procedure is described for the separation of membrane vesicles from Caulobacter crescentus cells into flagellated (polar) vesicles and nonflagellated (nonpolar) vesicles. Analysis by two-dimensional gel electrophoresis shows that a number of proteins are associated primarily with either the polar or the nonpolar fraction, and this result suggests that the envelope of these cells is organized into at least two relatively stable domains. Radioimmunoassay also shows that the membrane pool of flagellin, which is known to behave as a precursor in the assembly of the flagellar filament, may be localized exclusively in the polar membrane domain. Thus, the results provide biochemical evidence for the structural and functional differentiation of the C. crescentus cell envelope. These findings are consistent with a model we proposed previously to explain the targeting of surface structures to the new cell pole of C. crescentus. The immunoadsorption approach described here should be useful in the further investigation of this problem, as well as in the fractionation of membrane domains with characteristic surface antigens in other systems.