Silver staining as a tool for neurotoxic assessment.
Silver staining as a tool for neurotoxic assessment.
复制标题
银染色作为神经毒性评估的工具。
DOI：
10.1037/e495922006-007
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Záborszky,L
中科院分区：
文献类型：
--
作者：
Beltramino,CA;deOlmos,JS;Gallyas,F;Heimer,L;Záborszky,L
神经毒理学和神经毒性已成为公众高度关注的问题，人们加紧努力寻找可靠和方便的方法来识别那些由于对中枢神经系统（CNS）有毒性作用而对健康可能有害的化学物质和物质。以下问题需要一个可靠的答案：中枢神经系统的基本单位（即神经元）是否因毒性作用而遭受不可逆转的损害？换句话说，我们的任务是找到一种简便的方法来检测神经元变性的迹象，并确定中枢神经系统中神经元变性的位置和程度。还原银法过去一直是绘制实验诱导神经元变性图谱的最有效方法，现代银法所证明的结果表明，它们还具有使其成为筛选各种神经毒性物质（包括滥用药物）引起的神经元损伤的有力方法所必需的品质。自本世纪初以来，银法已成功用于对神经组织的正常和病理成分进行染色（von Fajersztajn 1901；Bielschowsky） 1904 年；拉姆·尼·卡哈尔 1904 年）。各种组织成分容易吸引银以及金属银沉积物提供的高对比度显微图像是银浸渍的两个最有吸引力的特征。
Neurotoxicology and neurotoxicity have become matters of great public concern, and efforts have intensified to find reliable and convenient methods to identify those chemicals and substances that are potentially harmful to health because of their toxic effect on the central nervous system (CNS). A reliable answer is needed for the following question: Have the basic units of the CNS (ie, the neurons) suffered irreversible damage as a result of the toxic effect? In other words, the task is to find a handy method to detect signs of degeneration in neurons and to determine the location and extent of such neuronal degeneration in the CNS. The reduced silver methods’ have in the past been the most effective procedures for mapping experimentally induced neuronal degenerations, and the results demonstrated by modern silver methods indicate that they also have the qualities necessary to make them a powerful method for screening neuronal damage caused by various neurotoxic substances including drugs of abuse.Since the early part of of this century, silver methods have been used with great success to stain both normal and pathologic components of nervous tissue (von Fajersztajn 1901; Bielschowsky 1904; Ram ny Cajal 1904). The ease with which various tissue components attract silver and the high-contrast microscopic images provided by metallic silver deposits are two of the most attractive features of silver impregnation.