Imbalance between endothelial progenitors cells and microparticles in HIV-infected patients naive for antiretroviral therapy
Imbalance between endothelial progenitors cells and microparticles in HIV-infected patients naive for antiretroviral therapy
复制标题
DOI：
10.1097/qad.0b013e32834980f4
复制
发表时间：
2011-08-24
期刊：
影响因子：
3.8
通讯作者：
Lewi, David S.
中科院分区：
文献类型：
--
作者：
da Silva, Erika F. R.;Fonseca, Francisco A. H.;Lewi, David S.
背景：在接受抗逆转录病毒治疗的HIV感染患者中，已有心血管事件的报道。然而，HIV本身在决定血管损伤中的作用却很少被描述。慢性炎症状态可能会损害某些调节性内皮特性，导致其活化、凋亡或侵蚀。目的：评估HIV感染抗逆转录病毒药物初治患者内皮祖细胞和微粒之间的平衡。设计：一项病例对照研究，将HIV感染患者（n = 35）与性别和年龄匹配的健康对照（n = 33）进行比较。方法：通过流式细胞术定量表达CD 34（+）、CD 133（+）和KDR+的内皮祖细胞群。还测量了表达CD 51（+）的内皮源性微粒和表达CD 31（+）/CD 42（+）的血小板源性微粒。结果：HIV感染者的内皮祖细胞（CD 34（+）/KDR+）的百分比低于对照组（0.02vs.0.09%，P = 0. 045）。此外，HIV感染者的内皮微粒浓度更高（1963 vs.436微粒/ml血小板贫乏血浆，P = 0.003），各组间血小板衍生微粒的数量相似。此外，HIV感染者的血流介导的血管扩张低于对照组[平均值（SEM）：分别为10（1）和16%（2; P = 0.03）]。结论：我们的研究结果表明内皮祖细胞动员和内皮细胞凋亡之间的不平衡。内皮细胞更新的改变可能导致HIV感染患者的心血管事件。(C)2011年威科健康|利平科特威廉姆斯&威尔金斯
Background: Cardiovascular events have been reported among HIV-infected patients following antiretroviral therapy. However, the role of HIV itself in determining vascular damage is less described. Chronic inflammatory state might impair some regulatory endothelium properties leading to its activation, apoptosis or erosion.Objectives: To evaluate the balance between endothelial progenitor cells and micro-particles in HIV-infected antiretroviral drug-naive patients.Design: A case-control study comparing HIV-infected patients (n = 35) with sex-matched and age-matched healthy controls (n = 33).Methods: Endothelial progenitor cells populations expressing CD34(+), CD133(+) and KDR+ were quantified by flow cytometry. Endothelial-derived microparticles, expressing CD51(+), and platelet-derived microparticles, expressing CD31(+)/CD42(+), were also measured. Endothelial function was estimated by flow-mediated dilation.Results: Lower percentages of endothelial progenitor cells (CD34(+)/KDR+) were observed in HIV-infected individuals compared with controls (0.02 vs. 0.09%, P = 0.045). In addition, endothelial microparticles concentration was higher in HIV-infected individuals (1963 vs. 436 microparticles/ml platelet-poor plasma, P = 0.003), with similar number of platelet-derived microparticles among groups. Furthermore, flow-mediated dilation was lower among HIV-infected individuals compared with controls [mean (SEM): 10 (1) and 16% (2), respectively; P = 0.03].Conclusion: Our findings suggest an imbalance between endothelial progenitor cells mobilization and endothelial apoptosis. The alteration in the turnover of endothelial cells may contribute to cardiovascular events in HIV-infected patients. (C) 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins