Characterization of PUD-1 and PUD-2, two proteins up-regulated in a long-lived daf-2 mutant.
Characterization of PUD-1 and PUD-2, two proteins up-regulated in a long-lived daf-2 mutant.
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DOI：
10.1371/journal.pone.0067158
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Dong MQ
中科院分区：
文献类型：
--
作者：
Ding YH;Du YG;Luo S;Li YX;Li TM;Yoshina S;Wang X;Klage K;Mitani S;Ye K;Dong MQ
C.在daf-2（功能丧失）（PUD）中上调的两种蛋白质--秀丽线虫PUD-1和PUD-2是同源的17-kD蛋白质，在胰岛素信号传导减少的长寿daf-2突变动物中具有大量增加。在这项研究中，我们发现PUD-1和PUD-2在肠和皮下组织中大量表达，并形成异二聚体。我们已经将它们的晶体结构解析到了1.9 μ m的分辨率，并发现这两种蛋白质在V形二聚体中采用了相似的β-夹心折叠。相反，它们的同源物PUD-3、PUD-4、PUDL-1和PUDL-2都是单体蛋白，在C.优雅因此，PUD-1/PUD-2异源二聚体可能具有不同于其家族成员的功能。pud-1和pud-2的过表达或缺失均不影响WT或daf-2突变动物的寿命，这表明它们在daf-2蠕虫中的诱导并不有助于长寿。奇怪的是，pud-1和pud-2的缺失与针对淀粉样β肽（1-42）诱导的麻痹的保护作用相关，这进一步增强了daf-2（RNAi）对Aβ的保护作用。
C. elegans PUD-1 and PUD-2, two proteins up-regulated in daf-2(loss-of-function) (PUD), are homologous 17-kD proteins with a large abundance increase in long-lived daf-2 mutant animals of reduced insulin signaling. In this study, we show that both PUD-1 and PUD-2 are abundantly expressed in the intestine and hypodermis, and form a heterodimer. We have solved their crystal structure to 1.9-Å resolution and found that both proteins adopt similar β-sandwich folds in the V-shaped dimer. In contrast, their homologs PUD-3, PUD-4, PUDL-1 and PUDL-2 are all monomeric proteins with distinct expression patterns in C. elegans. Thus, the PUD-1/PUD-2 heterodimer probably has a function distinct from their family members. Neither overexpression nor deletion of pud-1 and pud-2 affected the lifespan of WT or daf-2 mutant animals, suggesting that their induction in daf-2 worms does not contribute to longevity. Curiously, deletion of pud-1 and pud-2 was associated with a protective effect against paralysis induced by the amyloid β-peptide (1-42), which further enhanced the protection conferred by daf-2(RNAi) against Aβ.
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影响因子：
3.9
作者：
Murphy, Coleen T.
通讯作者：
Murphy, Coleen T.
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
3.4
作者：
Bantscheff, M;Ringel, B;Thiesen, HJ
通讯作者：
Thiesen, HJ
影响因子：
4.8
作者：
McElwee, JJ;Schuster, E;Gems, D
通讯作者：
Gems, D
影响因子：
2.7
作者：
Kirienko, Natalia V.;Fay, David S.
通讯作者：
Fay, David S.