Crystal structure of oligomeric β1-adrenergic G protein-coupled receptors in ligand-free basal state.
Crystal structure of oligomeric β1-adrenergic G protein-coupled receptors in ligand-free basal state.
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DOI：
10.1038/nsmb.2504
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发表时间：
2013-04
影响因子：
16.8
通讯作者：
Huang, Xin-Yun
中科院分区：
文献类型：
--
作者：
Huang, Jianyun;Chen, Shuai;Zhang, J. Jillian;Huang, Xin-Yun
G protein-coupled receptors (GPCRs) mediate transmembrane signaling. Before ligand binding, GPCRs exist in a basal state. Crystal structures of several GPCRs bound with antagonists or agonists have been solved. However, the crystal structure of the ligand-free basal state of a GPCR, the starting point of GPCR activation and function, has not been determined. Here we report the X-ray crystal structure of the first ligand-free basal state of a GPCR in a lipid membrane-like environment. Oligomeric turkey β1-adrenergic receptors display two alternating dimer interfaces. One interface involves the transmembrane domain (TM) 1, TM2, the C-terminal H8, and the extracellular loop 1. The other interface engages residues from TM4, TM5, the intracellular loop 2 and the extracellular loop 2. Structural comparisons show that this ligand-free state is in an inactive conformation. This provides the structural information regarding GPCR dimerization and oligomerization.
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影响因子：
3.6
作者：
Baker, Jillian G.;Proudman, Richard G. W.;Tate, Christopher G.
通讯作者：
Tate, Christopher G.
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
64.8
作者：
Choe, Hui-Woog;Kim, Yong Ju;Ernst, Oliver P.
通讯作者：
Ernst, Oliver P.
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
影响因子：
11.4
作者：
Fung, Juan Jose;Deupi, Xavier;Pardo, Leonardo;Yao, Xiao Jie;Velez-Ruiz, Gisselle A.;DeVree, Brian T.;Sunahara, Roger K.;Kobilka, Brian K.
通讯作者：
Kobilka, Brian K.