CONTROL OF THE METABOLIC FLUX IN A SYSTEM WITH HIGH ENZYME CONCENTRATIONS AND MOIETY-CONSERVED CYCLES - THE SUM OF THE FLUX CONTROL COEFFICIENTS CAN DROP SIGNIFICANTLY BELOW UNITY
CONTROL OF THE METABOLIC FLUX IN A SYSTEM WITH HIGH ENZYME CONCENTRATIONS AND MOIETY-CONSERVED CYCLES - THE SUM OF THE FLUX CONTROL COEFFICIENTS CAN DROP SIGNIFICANTLY BELOW UNITY
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DOI：
10.1111/j.1432-1033.1992.tb17402.x
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发表时间：
1992-11-15
期刊：
影响因子：
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通讯作者：
KURGANOV, BI
中科院分区：
文献类型：
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作者：
KHOLODENKO, BN;LYUBAREV, AE;KURGANOV, BI
在许多代谢途径中，酶的浓度与底物的浓度相当。最近已经表明，如果这样的系统包含部分保守的循环，则违反“经典”代谢控制理论的许多陈述。对于任意路径，我们发现：(a) 连接系数 C(Ei)J（通过改变 E(i) 浓度获得）和 C(vi)J（通过改变 k(i)cat 获得）的方程，以及 (b) 修改的求和方程。在所考虑的系统中，酶控制系数的总和（等于“经典”理论下的统一）似乎总是低于统一。通过系数 C(Ei)J 之和达到负值的数值示例说明了所揭示的关系。提出了一种对上述系数进行实验测量的方法。
In a number of metabolic pathways enzyme concentrations are comparable to those of substrates. Recently it has been shown that many statements of the 'classical' metabolic control theory are violated if such a system contains a moiety-conserved cycle. For arbitrary pathways we have found: (a) the equation connecting coefficients C(Ei)J (obtained by varying the E(i) concentration) and C(vi)J (obtained by varying the k(i)cat), and (b) modified summation equations. The sum of the enzyme control coefficients (equal to unity under the 'classical' theory) appears always to be below unity in the systems considered. The relationships revealed were illustrated by a numerical example where the sum of coefficients C(Ei)J reached negative values. A method for experimental measurements of the above coefficients is proposed.