Roles of the novel coiled-coil protein Rng10 in septum formation during fission yeast cytokinesis.
Roles of the novel coiled-coil protein Rng10 in septum formation during fission yeast cytokinesis.
复制标题
裂变酵母细胞球运动期间，新型卷曲螺旋蛋白RNG10在隔膜形成中的作用。
DOI：
10.1091/mbc.e16-03-0156
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发表时间：
2016-08-15
影响因子：
3.3
通讯作者：
Wu JQ
中科院分区：
文献类型：
--
作者：
Liu Y;Lee IJ;Sun M;Lower CA;Runge KW;Ma J;Wu JQ
Rho-Gap局部化的调节机制还不是很清楚。一种新的螺旋卷曲蛋白RNG10的特征是定位于裂解酵母中的Rho-GAP RGa7。RNG10和RGa7在细胞质分裂过程中相互作用，共同调节葡聚糖合成酶的积累和动态，从而成功地形成隔膜。Rho间隙是Rho GTP酶的重要调节因子，参与胞质分裂和其他过程的各个步骤。然而，对Rho-GAP细胞定位和功能的调控还不完全清楚。在这里，我们报道了一种新的螺旋卷曲蛋白RNG10的特征及其与裂解酵母中Rho-GAP RGa7的关系。在胞质分裂过程中，rNG10Δ和rGa7Δ都会导致隔膜缺陷和细胞溶解。RNG10和RGa7共定位于间期细胞顶端的质膜和细胞分裂时的分裂部位。RNG10在亲和纯化和免疫共沉淀中与RGa7发生物理相互作用。有趣的是，如果没有RNG10，Rga7本地化几乎被废除。此外，RNG10和RGa7共同调节葡聚糖合成酶的积累和动态，从而在细胞质分裂中成功地形成隔膜。我们的结果表明，在分裂酵母的胞质分裂过程中，Rho-GAP RGa7的细胞定位和功能受到一种新的蛋白质RNG10的调节。
The regulation of Rho-GAP localization is not well understood. A novel coiled-coil protein Rng10 is characterized that localizes the Rho-GAP Rga7 in fission yeast. Rng10 and Rga7 physically interact and work together to regulate the accumulation and dynamics of glucan synthases for successful septum formation during cytokinesis. Rho GAPs are important regulators of Rho GTPases, which are involved in various steps of cytokinesis and other processes. However, regulation of Rho-GAP cellular localization and function is not fully understood. Here we report the characterization of a novel coiled-coil protein Rng10 and its relationship with the Rho-GAP Rga7 in fission yeast. Both rng10Δ and rga7Δ result in defective septum and cell lysis during cytokinesis. Rng10 and Rga7 colocalize on the plasma membrane at the cell tips during interphase and at the division site during cell division. Rng10 physically interacts with Rga7 in affinity purification and coimmunoprecipitation. Of interest, Rga7 localization is nearly abolished without Rng10. Moreover, Rng10 and Rga7 work together to regulate the accumulation and dynamics of glucan synthases for successful septum formation in cytokinesis. Our results show that cellular localization and function of the Rho-GAP Rga7 are regulated by a novel protein, Rng10, during cytokinesis in fission yeast.