The Fate of the Adipose-Derived Stromal Cells during Angiogenesis and Adipogenesis after Cell-Assisted Lipotransfer
The Fate of the Adipose-Derived Stromal Cells during Angiogenesis and Adipogenesis after Cell-Assisted Lipotransfer
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DOI：
10.1097/prs.0000000000004021
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发表时间：
2018-02-01
影响因子：
3.6
通讯作者：
Minn, Kyung Won
中科院分区：
文献类型：
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作者：
Hong, Ki Yong;Yim, Sangjun;Minn, Kyung Won
背景：细胞辅助脂肪移植是一种脂肪移植中加入自体脂肪基质细胞的方法。由于该技术的有效性得到证实，研究集中在细胞辅助脂肪转移提高移植物存活率的机制上。然而，与细胞辅助lipotransfer.Methods：作者介绍了一种动物模型，细胞辅助lipotransfer使用两种不同的转基因报告小鼠的供体和受体组织的微环境变化仍然不清楚。将来自表达绿色荧光蛋白的C57 BL/6 J小鼠的供体脂肪和来自表达DsRed的C57 BL/6 J小鼠的供体脂肪来源的基质细胞共移植到受体C57 BL/6 J小鼠中。在脂肪重塑后，细胞辅助lipotransfer，每个捐助者的脂肪细胞和捐助者脂肪来源的基质细胞的命运进行了跟踪使用免疫荧光染色与whole mount method.Results：脂肪来源的基质细胞补充改变炎症，促进血管生成和随后的受体组织中的血管重建。术后第4周追踪显示，存活的供体脂肪源性基质细胞通过分化为内皮细胞参与血管生成。此外，来自供体脂肪来源的基质细胞和受体组织的新分化的脂肪与存活的供体脂肪整合，从而改善移植物的保留。脂肪来源的基质细胞补充导致在血管生成和脂肪生成的定量差异在脂肪remodeling根据脂肪来源的基质cells.Conclusions的浓度：作者的特点是发生在供体脂肪来源的基质细胞和脂肪和受体组织的动态变化，通过跟踪这些细胞成分后，细胞辅助lipotransfer。作者的发现强调了细胞辅助脂肪移植在组织移植中的治疗价值。
Background: Cell-assisted lipotransfer is a process in which fat grafting is supplemented with autologous adipose-derived stromal cells. Since the efficacy of the technique was demonstrated, studies have focused on the mechanism by which cell-assisted lipotransfer enhances the rate of graft survival. However, the microenvironmental changes in donor and recipient tissue associated with cell-assisted lipotransfer remain unclear.Methods: The authors introduced an animal model of cell-assisted lipotransfer using two different transgenic reporter mice. Donor fat from green fluorescent protein-expressing C57BL/6J mice and donor adipose-derived stromal cells from DsRed-expressing C57BL/6J mice were co-transplanted into recipient C57BL/6J mice. During adipose remodeling after cell-assisted lipotransfer, the fate of each donor adipocyte and donor adipose-derived stromal cell was traced using immunofluorescent staining with the whole-mount method.Results: Adipose-derived stromal cell supplementation altered inflammation and promoted angiogenesis and subsequent revascularization in recipient tissue. Tracing at postoperative week 4 revealed that surviving donor adipose-derived stromal cells participated in angiogenesis by differentiating into endothelial cells. Moreover, newly differentiated fat from donor adipose-derived stromal cells and recipient tissue integrated with surviving donor fat, leading to improved retention of the graft. Adipose-derived stromal cell supplementation resulted in a quantitative difference in angiogenesis and adipogenesis during adipose remodeling according to the concentration of adipose-derived stromal cells.Conclusions: The authors characterized the dynamic changes occurring in donor adipose-derived stromal cells and fat and recipient tissue by tracing these cellular components following cell-assisted lipotransfer. The authors' findings highlight the therapeutic value of cell-assisted lipotransfer in tissue transplantation.