Odor detection ability and thallium-201 transport in the olfactory nerve of traumatic olfactory-impaired mice
Odor detection ability and thallium-201 transport in the olfactory nerve of traumatic olfactory-impaired mice
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DOI：
10.1093/chemse/bjn030
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发表时间：
2008-09-01
期刊：
影响因子：
3.5
通讯作者：
Miwa, Takaki
中科院分区：
文献类型：
--
作者：
Shiga, Hideaki;Kinoshita, Yayoi;Miwa, Takaki
虽然嗅神经损伤是诊断创伤后嗅觉丧失的一个因素，但目前还没有直接评估这些神经损伤的方法。我们已经表明，在小鼠嗅神经损伤后，铊-201（Tl-201）从鼻腔到嗅球的转运减少。为了确定神经损伤后的嗅觉功能是否可以通过鼻内施用Tl-201来评估，我们测量了气味检测能力（ODA）和Tl-201在嗅神经中的转运速率之间的相关性。ODA和TI-201运输后，双侧嗅神经横断4周的时间内进行了测量。将环己酰亚胺溶液用于相对于自来水的ODA。Tl-201转运用γ光谱法测量为鼻腔和嗅球中的放射性比率。ODA与Tl-201在嗅神经中的转运率之间存在显著相关性。这些发现表明，鼻内给予Tl-201可以客观地评价神经损伤后的嗅觉功能。
Although olfactory nerve damage is a contributing factor in the diagnosis of posttraumatic olfactory loss, at present, there are no methods to directly assess injury to these nerves. We have shown that following olfactory nerve injury in mice, thallium-201 (Tl-201) transport from the nasal cavity to the olfactory bulb decreases. To determine if olfactory function after nerve injury could be assessed with nasal administration of Tl-201, we measured the correlation between odor detection ability (ODA) and the rate of transport of Tl-201 in olfactory nerves. Both ODA and Tl-201 transport were measured after bilateral olfactory nerve transection for a 4-week period. Cycloheximide solution was used for ODA against tap water. Tl-201 transport was measured as the ratio of radioactivity in the nasal cavity and olfactory bulb with gamma spectrometry. There was a significant correlation between ODA and the rate of Tl-201 transport in the olfactory nerve. These findings suggest that olfactory function after nerve injury can be objectively evaluated with the nasal administration of Tl-201.