Paeoniflorin inhibits nucleus pulposus cell apoptosis by regulating the expression of Bcl-2 family proteins and caspase-9 in a rabbit model of intervertebral disc degeneration
Paeoniflorin inhibits nucleus pulposus cell apoptosis by regulating the expression of Bcl-2 family proteins and caspase-9 in a rabbit model of intervertebral disc degeneration
复制标题
DOI：
10.3892/etm.2015.2501
复制
发表时间：
2015-07-01
影响因子：
2.7
通讯作者：
Wang, Jing
中科院分区：
文献类型：
--
作者：
Shi, Lijun;Teng, Honglin;Wang, Jing
细胞凋亡在椎间盘内破裂（IDD）的发病机制中起重要作用，因此，抑制细胞凋亡可能为IDD疾病的治疗提供新的途径。本研究旨在通过检测芍药苷对碘缺乏病家兔模型相关指标的影响，探讨芍药苷的作用及其机制。研究中总共使用了144只家兔，并分为4组（每组n=36）。造模成功的家兔分别灌胃给予芍药苷120 mg/kg·d（高剂量组）、30 mg/kg·d（低剂量组）或生理盐水（模型盐水组），非IDD家兔作为正常对照组。流式细胞仪检测椎间盘髓核细胞凋亡率。采用免疫组化和western blot法检测治疗前后椎间盘组织中Bcl-2、Bax和caspase-9的表达水平。结果表明，芍药苷低、高剂量组与模型组比较，Bax表达水平明显降低，Bcl-2表达水平明显升高（P
Apoptosis plays a key role in the pathogenesis of internal disc disruption (IDD); therefore, the inhibition of apoptosis may offer a novel approach for treating IDD diseases. The aim of the present study was to investigate the effects and the underlying mechanisms of paeoniflorin through the detection of relevant indicators in a rabbit model of IDD. In total, 144 rabbits were used in the study and divided into four groups (n=36 per group). Rabbits successfully modeled with IDD received an intragastric injection of 120 mg/kgday paeoniflorin (high-dose group), 30 mg/kgday paeoniflorin (low-dose group) or saline (model saline group), while rabbits without IDD were used as a normal control group. The apoptosis rate of disc nucleus pulposus cells was detected using flow cytometry. In addition, the expression levels of Bcl-2, Bax and caspase-9 in the disc tissues were detected using immunohistochemistry and western blot analysis prior to and following the treatment. The results indicated that the expression levels of Bax in the low- and high-dose paeoniflorin groups were significantly reduced, while the Bcl-2 expression levels were significantly increased when compared with the model saline group (P