Physical factors affecting kinesin-based transport of synthetic nanoparticle cargo
Physical factors affecting kinesin-based transport of synthetic nanoparticle cargo
复制标题
DOI：
10.1166/jnn.2005.112
复制
发表时间：
2005-05-01
影响因子：
--
通讯作者：
Bachand, GD
中科院分区：
文献类型：
--
作者：
Bachand, M;Trent, AM;Bachand, GD
最近，驱动蛋白生物分子马达和微管细丝（MT）被用于运输金属和半导体纳米颗粒，长期目标是利用这种主动运输系统来动态组装纳米结构材料。然而，在某些情况下，MT上纳米颗粒货物的存在显示通过干扰驱动蛋白-MT相互作用来抑制转运。这项工作的主要目标是（1）确定哪些因素影响驱动蛋白和MT运输纳米颗粒货物的能力，以及（2）建立一个功能参数空间，其中驱动蛋白和MT可以支持纳米颗粒和材料的无阻碍运输。在评估的因素中，给定MT上的纳米颗粒密度是影响基于驱动蛋白的纳米颗粒转运的最重要因素。通过限制可用的连接位点的数目来控制颗粒的密度（即，生物素化微管蛋白）和/或溶液中纳米颗粒的相对浓度。纳米颗粒大小也是影响转运的重要因素，并归因于颗粒的能力
Recently, kinesin biomolecular motors and microtubules filaments (MTs) were used to transport metal and semiconductor nanoparticles with the long-term goal of exploiting this active transport system to dynamically assemble nanostructured materials. In some cases, however, the presence of nanoparticle cargo on MTs was shown to inhibit transport by interfering with kinesin-MT interactions. The primary objectives of this work were (1) to determine what factors affect the ability of kinesin and MTs to transport nanoparticle cargo, and (2) to establish a functional parameter space in which kinesin and MTs can support unimpeded transport of nanoparticles and materials. Of the factors evaluated, nanoparticle density on a given MT was the most significant factor affecting kinesin-based transport of nanoparticles. The density of particles was controlled by limiting the number of available linkage sites (i.e., biotinylated tubulin), and/or the relative concentration of nanoparticles in solution. Nanoparticle size was also a significant factor affecting transport, and attributed to the ability of particles