Internal concentrations of perfluorobutane sulfonate (PFBS) comparable to those of perfluorooctane sulfonate (PFOS) induce reproductive toxicity in Caenorhabditis elegans
Internal concentrations of perfluorobutane sulfonate (PFBS) comparable to those of perfluorooctane sulfonate (PFOS) induce reproductive toxicity in Caenorhabditis elegans
复制标题
全氟丁磺酸 (PFBS) 的内部浓度与全氟辛烷磺酸 (PFOS) 相当，可引起秀丽隐杆线虫的生殖毒性
DOI：
10.1016/j.ecoenv.2018.04.032
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发表时间：
2018-08-30
影响因子：
6.8
通讯作者：
Liang, Yong
中科院分区：
文献类型：
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作者：
Chen, Fengjie;Wei, Cuiyun;Liang, Yong
全氟丁烷磺酸（PFBS）被认为是全氟辛烷磺酸（PFOS）的毒性较低的替代品，在工业和消费品中有多种应用。以前比较它们毒性的研究通常使用相似的暴露水平，而不考虑内部浓度。目前的研究比较了内部浓度相似的全氟辛烷磺酸和全氟辛烷磺酸对秀丽隐杆线虫（秀丽隐杆线虫）的生殖毒性。全氟辛烷磺酸的生物蓄积性远低于全氟辛烷磺酸。全氟辛烷磺酸和全氟苯磺酸的48 h中位致死浓度（LC50）分别为1.4 μ M（95%可信区间[CI]: 1.1 ~ 1.6）和794 μ M （95% CI: 624 ~ 1009）。暴露于0.1 μ M全氟辛烷磺酸或1000 μ M、1500 μ M全氟辛烷磺酸后，秀丽隐杆线虫的产蛋量和产卵数显著下降。暴露于2 μ M全氟辛烷磺酸或500或1000 μ M全氟辛烷磺酸后，生殖细胞凋亡和活性氧的产生显著增加。PFOS和PFBS暴露后，抗氧化基因sod-3、ctl-2和gst-4以及促凋亡基因egl-1和ced-13的表达显著改变。这些发现表明，这两种化学物质对秀丽隐杆线虫具有生殖毒性，可能是由于活性氧水平升高导致生殖细胞凋亡。本研究中使用的全氟辛烷磺酸和全氟辛烷磺酸的暴露浓度差异很大，但产生了相似的内部浓度，导致观察到的生殖毒性。
Perfluorobutane sulfonate (PFBS) is considered a less-toxic replacement for perfluorooctane sulfonate (PFOS), with multiple applications in industrial and consumer products. Previous studies comparing their toxicity generally used similar exposure levels, without taking internal concentrations into account. The current study compared the reproductive toxicity of PFOS and PFBS, at similar internal concentrations, to Caenorhabditis elegans (C. elegans). PFBS was much less bioaccumulative than PFOS. The 48-h median lethal concentrations (LC50) for PFOS and PFBS were 1.4 mu M (95% confidence interval [CI): 1.1-1.6) and 794 mu M (95% CI: 624-1009), respectively. Egg production and brood number of C. elegans decreased markedly following exposure to 0.1 mu M PFOS or 1000 or 1500 mu M PFBS. Germ-cell apoptosis and production of reactive oxygen species increased significantly following exposure to 2 mu M PFOS or 500 or 1000 mu M PFBS. Expression of the antioxidant genes sod-3, ctl-2, and gst-4 and the pro-apoptotic genes egl-1 and ced-13 was altered significantly following PFOS and PFBS exposure. These findings indicate that both chemicals exert reproductive toxicity in C. elegans, probably owing to germ-cell apoptosis resulting from elevated levels of reactive oxygen species. The vastly different exposure concentrations of PFBS and PFOS used in this study produced similar internal concentrations, leading to the reproductive toxicities observed.