Ability of antioxidants eliminates the peroxyl radical derived from 2,-27-azobis(2-amidinopropane) dihydrochloride (AAPH)
Ability of antioxidants eliminates the peroxyl radical derived from 2,-27-azobis(2-amidinopropane) dihydrochloride (AAPH)
复制标题
抗氧化剂能够消除源自 2,-27-偶氮双(2-脒基丙烷)二盐酸盐 (AAPH) 的过氧自由基
DOI：
10.1007/s00723-011-0227-3
复制
发表时间：
2014
影响因子：
1
通讯作者：
T. Ikenoue,
中科院分区：
文献类型：
--
作者：
A. Nakajima;Y. Sakurai;E. Matsuda;Y. Masuda;Y. Naganobu;K. Tajima;H. Sameshima;T. Ikenoue,
建立了一种新的高效液相色谱（HPLC）-电子自旋共振（ESR）自旋捕集柱后在线检测超氧自由基清除活性的方法。用环状硝酮型自旋捕获剂5，5-二甲基-1-吡咯啉-N-氧化物（DMPO）检测核黄素依赖的光还原氧分子产生的超氧自由基。通过监测DMPO自旋加合物的ESR信号强度，优化了反应条件。记录标准样品溶液的HPLC-ESR色谱图，所述标准样品溶液包括三种酚类化合物，没食子酸（Gal）、4-羟基香豆酸（Cou）和咖啡酸（Caf）。
A new high-performance liquid chromatography (HPLC)–electron spin resonance (ESR) spin-trapping system for post-column on-line detection of the superoxide radical scavenging activity of column eluates was developed. A cyclic nitrone-type spin-trapping reagent, 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), was used for detection of the superoxide radical, which was produced by riboflavin-dependent photo-reduction of molecular oxygen in the presence of an electron donor. The reaction conditions were optimized by monitoring the ESR signal intensity of the spin-adduct of DMPO. HPLC–ESR chromatographs were recorded for the standard sample solution, which included three phenolic compounds, gallic acid (Gal), 4-hydroxycoumaric acid (Cou), and caffeic acid (Caf).