High-throughput methods for visualizing the teleost skeleton: capturing autofluorescence of alizarin red
High-throughput methods for visualizing the teleost skeleton: capturing autofluorescence of alizarin red
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DOI：
10.1111/j.1439-0426.2010.01419.x
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发表时间：
2010-04-01
影响因子：
0.9
通讯作者：
Yelick, P. C.
中科院分区：
文献类型：
--
作者：
Connolly, M. H.;Yelick, P. C.
近一个世纪以来，形态学家一直使用茜素红S（茜素磺酸钠）对钙化骨基质进行染色。最近，其传统用途经常被更现代的技术所取代。然而，其自身荧光特性继续为骨骼发育和再生以外的研究做出贡献。本研究的目的是描述可用于捕获幼年斑马鱼茜素红染色矿化组织的自发荧光的详细方法。这些方法允许对微小骨骼元素（例如咽齿）进行原位分析，并且不需要解剖。
P>For nearly a century, Alizarin red S (alizarin sodium sulfonate) has been used by morphologists to stain calcified bone matrix. More recently, its traditional use has frequently been replaced by more modern techniques; however, its auto fluorescent property continues to contribute to research well beyond the context of bone development and regeneration. The purpose of this study is to describe detailed methods that can be used to capture the autofluorescence of Alizarin red-stained mineralized tissues in juvenile zebrafish. These methods allow for in situ analyses of minute skeletal elements, such as pharyngeal teeth, and preclude the need for dissection.