Olig2-positive progenitors in the embryonic spinal cord give rise not only to motoneurons and oligodendrocytes, but also to a subset of astrocytes and ependymal cells
Olig2-positive progenitors in the embryonic spinal cord give rise not only to motoneurons and oligodendrocytes, but also to a subset of astrocytes and ependymal cells
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DOI：
10.1016/j.ydbio.2006.02.029
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发表时间：
2006-05-15
影响因子：
2.7
通讯作者：
Ikenaka, Kazuhiro
中科院分区：
文献类型：
--
作者：
Masahira, Noritaka;Takebayashi, Hirohide;Ikenaka, Kazuhiro
胚胎脊髓中的运动神经元和少突胶质细胞是由腹侧脑室带的一个受限区域产生的，称为pMN区域。pMN结构域是两个基本的螺旋-环-螺旋转录因子Olig1和Olig2的表达位点，它们对运动神经元和少突胶质细胞的发育至关重要。先前使用Olig1-Cre和Olig2-GFP小鼠进行的谱系追踪实验表明，运动神经元和少突胶质细胞，而不是星形胶质细胞，是由pMN结构域产生的。然而，重要的问题仍然存在，包括pMN结构域内神经上皮细胞的命运，特别是运动神经元和少突胶质细胞是否是pMN结构域内唯一产生的细胞类型。我们使用他莫昔芬诱导的cree -recombinase插入Olig2位点进行了谱系追踪实验。我们证明运动神经元和少突胶质细胞祖细胞来源于pMN结构域的Olig2（+）祖细胞，并且还发现脊髓腹侧表面的星形胶质细胞亚群和脑室表面的室管膜细胞也来自pMN结构域。这些发现表明，运动神经元和少突胶质细胞不是起源于该区域的唯一细胞类型。(c) 2006爱思唯尔公司版权所有。
Motoneurons and oligodendrocytes in the embryonic spinal cord are produced from a restricted domain of the ventral ventricular zone, termed the pMN domain. The pMN domain is the site of expression of two basic helix-loop-helix transcription factors, Olig1 and Olig2, which are essential for motoneuron and oligodendrocyte development. Previous lineage-tracing experiments using Olig1-Cre and Olig2-GFP mice suggested that motoneurons and oligodendrocytes, but not astrocytes, are produced from the pMN domain. However, important questions remain, including the fate of neuroepithelial cells in the pMN domain, and specifically whether motoneurons and oligodendrocytes are the only types of cells produced in the pMN domain. We performed lineage-tracing experiments using a tamoxifen-inducible Cre-recombinase inserted into the Olig2 locus. We demonstrated that motoneurons and oligodendrocyte progenitors are derived from the Olig2(+) progenitors in the pMN domain, and also found that a subset of astrocytes at the ventral surface of the spinal cord and ependymal cells at the ventricular surface are also produced from the pMN domain. These findings demonstrate that motoneurons and oligodendrocytes are not the only cell types originating from this domain. (c) 2006 Elsevier Inc. All rights reserved.