Influence of Advanced Age on Mycobacterium bovis BCG Vaccination in Guinea Pigs Aerogenically Infected with Mycobacterium tuberculosis
Influence of Advanced Age on Mycobacterium bovis BCG Vaccination in Guinea Pigs Aerogenically Infected with Mycobacterium tuberculosis
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DOI：
10.1128/cvi.00190-10
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发表时间：
2010-10-01
影响因子：
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通讯作者：
Yamamoto, Saburo
中科院分区：
文献类型：
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作者：
Komine-Aizawa, Shihoko;Yamazaki, Toshio;Yamamoto, Saburo
卡介苗（BCG）是目前唯一可用的结核病（TB）疫苗，但其对成人肺结核的疗效仍存在争议。BCG诱导针对分枝杆菌抗原的特异性免疫应答，并可能引发针对TB的保护性免疫。结核病仍然是一个主要的公共卫生问题，特别是在老年人中，但卡介苗在老年人中的疗效尚不清楚。我们研究了卡介苗接种预防年轻（6周龄）、中年（18月龄）和老年（60月龄）豚鼠结核病的能力。BCG东京疫苗接种减少了结核分枝杆菌H37 Rv在所有三组中的生长。通过使用酶联免疫斑点（ELISPOT）试验，在用BCG东京加强接种后的60月龄豚鼠中检测抗原特异性γ干扰素（IFN-γ）产生细胞。我们的研究结果表明，东京卡介菌对结核病感染有保护作用，无论年龄大小。
Mycobacterium bovis bacillus Calmette-Guerin (BCG) is the only tuberculosis (TB) vaccine currently available, but its efficacy against adult pulmonary TB remains controversial. BCG induces specific immune responses to mycobacterial antigens and may elicit protective immunity against TB. TB remains a major public health problem, especially among the elderly, yet the efficacy of BCG in the elderly is unknown. We investigated the ability of BCG vaccination to prevent TB in young (6-week-old), middle-aged (18-month-old), and old (60-month-old) guinea pigs. BCG-Tokyo vaccination reduced the growth of Mycobacterium tuberculosis H37Rv in all three groups. By use of an enzyme-linked immunospot (ELISPOT) assay, antigen-specific gamma interferon (IFN-gamma)-producing cells were detected in the 60-month-old guinea pigs after a booster vaccination with BCG-Tokyo. Our findings suggest that BCG-Tokyo has a protective effect against tuberculosis infection regardless of age.