Gut microbiota dysbiosis is associated with. Henoch-Schonlein Purpura in children
Gut microbiota dysbiosis is associated with. Henoch-Schonlein Purpura in children
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肠道菌群失调与儿童过敏性紫癜有关
DOI：
10.1016/j.intimp.2018.03.003
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发表时间：
2018-05-01
影响因子：
5.6
通讯作者：
Gai, Zhongtao
中科院分区：
文献类型：
--
作者：
Wang, Xingcui;Zhang, Lei;Gai, Zhongtao
背景资料：肠道微生物群的改变与过敏性疾病的发展有关，如哮喘和食物过敏。然而，目前还没有报道详细的作用，菌群的变化在过敏性紫癜（HSP）的development.Method：共85名儿童过敏性紫癜和70名健康儿童招募了这项研究。通过基于16S rRNA基因的焦磷酸测序分析肠道微生物群组成。结果：我们比较了155名受试者的肠道微生物群，发现HSP儿童表现出肠道微生物生态失调。与对照组相比，HSP患者的微生物多样性和丰富度较低。根据相似性分析，HSP患者的微生物群组成也与对照组不同（r = 0.306，P = 0.001）。过敏性紫癜患儿小杆菌属（P < 0.0001）、罗斯拜瑞氏菌属（P < 0.0001）和副小杆菌属（P < 0.0001）的相对丰度显著降低，而副拟杆菌属（P < 0.006）和肠球菌属（P < 0.0001）的相对丰度显著升高。斯皮尔曼相关分析表明，LOS与拟青霉属和罗斯拜瑞氏菌属呈显著负相关（P < 0.05）。同时，伊加水平与双歧杆菌属呈显著负相关（P < 0.01）。结论：HSP与肠道菌群组成和结构的显著变化有关。这些结果增强了未来基于微生物的疗法改善儿童HSP临床结局的潜力。
Background: Alterations in the intestinal microbiota have been associated with the development of allergic diseases, such as asthma and food allergies. However, there is no report detailing the role of microbiota alterations in Henoch-Schonlein Purpura (HSP) development.Method: A total of 85 children with HSP and 70 healthy children were recruited for this study. Intestinal microbiota composition was analyzed by 16S rRNA gene-based pyrosequencing. Fecal microbial diversity and composition were compared.Result: We compared the gut microbiota of 155 subjects and found that children with HSP exhibited gut microbial dysbiosis. Lower microbial diversity and richness were found in HSP patients when compared to the control group. Based on an analysis of similarities, the composition of the microbiota in HSP patients was also different from that of the control group (r = 0.306, P = 0.001). The relative abundance of the bacterial genera Dialister (P < 0.0001), Roseburia (P < 0.0001), and Parasutterella (P < 0.0001) was significantly decreased in HSP children, while the relative abundance of Parabacteroides (P < 0.006) and Enterococcus (P < 0.0001) in these children was significantly increased. Based on Spearman correlation analysis, the LOS showed a significant negative (P < 0.05) correlation with the genera Paraprevotella and Roseburia. Meanwhile, IgA levels exhibited a significant negative (P < 0.01) correlation with the genus Bifidobacterium.Conclusions: Our results indicate that HSP is associated with significant compositional and structural changes in the gut microbiota. These results enhance the potential for future microbial-based therapies to improve the clinical outcome of HSP in children.