Cucurbitacin B inhibits STAT3 and the Raf/MEK/ERK pathway in leukemia cell line K562
Cucurbitacin B inhibits STAT3 and the Raf/MEK/ERK pathway in leukemia cell line K562
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DOI：
10.1016/j.canlet.2009.07.015
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发表时间：
2010-03-01
期刊：
影响因子：
9.7
通讯作者：
Xie, Wei Dong
中科院分区：
文献类型：
--
作者：
Chan, Kin Tak;Li, Kwan;Xie, Wei Dong
葫芦素B是葫芦科植物中发现的一种天然抗癌化合物。葫芦素B对人白血病细胞的抗肿瘤作用已有报道，但其作用机制尚不清楚。为了阐明其抗癌活性和作用机制，用葫芦素B处理国家癌症研究所小组的五种不同的白血病细胞系（CCRF-CEM、K562、MOLT-4、RPMI-8226和SR）。葫芦素B对白血病细胞生长有抑制作用，其GI（50）为15.6 ~ 35.3 nM，其生长抑制作用主要是由于G2/M期阻滞和凋亡。细胞信号分子的Western印迹分析表明葫芦素B抑制K562细胞中STAT 3的活化和Raf/MEK/ERK通路。皇冠版权所有（C）2009由爱思唯尔爱尔兰有限公司出版。保留所有权利。
Cucurbitacin B is a natural anti-cancer compound found in Cucurbitaceae. Although the anti-cancer activity of cucurbitacin B in human leukemia cells has been reported, the underlining mechanism is still unclear. To clarify its anti-cancer activity and the mechanism of action, five different leukemia cell lines (CCRF-CEM, K562, MOLT-4, RPMI-8226 and SR) of the National Cancer Institute panel were treated with cucurbitacin B. Leukemia cell growth was inhibited by cucurbitacin B with GI(50) ranged from 15.6 nM to 35.3 nM and the growth inhibition effect was attributable to G2/M phase arrest and apoptosis. Western blotting analysis of cell signaling molecules indicated that cucurbitacin B inhibits STAT3 activation and the Raf/MEK/ERK pathway in the K562 cells. Crown Copyright (C) 2009 Published by Elsevier Ireland Ltd. All rights reserved.