Intraspecific DNA methylation polymorphism in cotton (Gossypium hirsutum L.)
Intraspecific DNA methylation polymorphism in cotton (Gossypium hirsutum L.)
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DOI：
10.1093/jhered/esl023
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发表时间：
2006-09-01
影响因子：
3.1
通讯作者：
Wendel, Jonathan F.
中科院分区：
文献类型：
--
作者：
Keyte, Anna L.;Percifield, Ryan;Wendel, Jonathan F.
胞嘧啶甲基化在植物基因表达和发育的表观遗传调控中是重要的，并且在几个植物组中在多倍体形成后涉及沉默重复基因。相对较少的信息存在，但是，在物种内同源基因座的甲基化多态性（MP）的水平和模式。在这里，我们探讨了在异源四倍体棉花，陆地棉CCGG位点的甲基化多态性多样性的水平和模式，使用甲基化敏感的扩增片段长度多态性筛选和一组选定的20 G。我们拥有有关遗传多态性水平和关系的信息的陆地植物品种。甲基化和MP在G. hirsutum：调查的150个HpaII/MspI位点中，48个在内部胞嘧啶甲基化（32%），其中32个是多态性的（67%）。这两个值都高于使用限制性片段长度多态性的遗传多样性的可比措施。甲基化多态性位点的高比例和与基因表达的潜在关系强调了MP在种群内和种群间的潜在意义。我们推测，棉花甲基化多态性位点和基因的偏相关性可能是多倍体和所有基因的伴随加倍的结果。
Cytosine methylation is important in the epigenetic regulation of gene expression and development in plants and has been implicated in silencing duplicate genes after polyploid formation in several plant groups. Relatively little information exists, however, on levels and patterns of methylation polymorphism (MP) at homologous loci within species. Here we explored the levels and patterns of methylation-polymorphism diversity at CCGG sites within allotetraploid cotton, Gossypium hirsutum, using a methylation-sensitive amplified fragment length polymorphism screen and a selected set of 20 G. hirsutum accessions for which we have information on genetic polymorphism levels and relationships. Methylation and MP exist at high levels within G. hirsutum: of 150 HpaII/MspI sites surveyed, 48 were methylated at the inner cytosine (32%) and 32 of these were polymorphic (67%). Both these values are higher than comparable measures of genetic diversity using restriction fragment length polymorphisms. The high percentage of methylation-polymorphic sites and potential relationship to gene expression Underscore the potential significance of MP within and among populations. We speculate that biased correlation of methylation-polymorphic sites and genes in cotton may be a consequence of polyploidy and the attendant doubling of all genes.