Sugary Kefir Strain Lactobacillus mali APS1 Ameliorated Hepatic Steatosis by Regulation of SIRT-1/Nrf-2 and Gut Microbiota in Rats
Sugary Kefir Strain Lactobacillus mali APS1 Ameliorated Hepatic Steatosis by Regulation of SIRT-1/Nrf-2 and Gut Microbiota in Rats
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DOI：
10.1002/mnfr.201700903
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发表时间：
2018-04-01
影响因子：
5.2
通讯作者：
Chen, Ming-Ju
中科院分区：
文献类型：
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作者：
Chen, Yung-Tsung;Lin, Yu-Chun;Chen, Ming-Ju
范围：非酒精性脂肪性肝病（NAFLD）是一种常见的疾病，伴随肥胖，导致死亡率增加。到目前为止，NAFLD治疗的效率仍有待提高。因此，我们的目的是评估苹果乳杆菌APS 1，这是从含糖开菲尔分离，对大鼠肝脏脂肪变性喂高脂饮食（HFD）的影响。方法和结果：Sprague道利大鼠喂对照饮食，HFD与生理盐水，和HFD与APS 1干预每天管饲12周。结果显示，APS 1显著降低了HFD喂养大鼠的体重和体重增加。APS 1通过调节SIRT-1/PGC-1 α/SREBP-1表达减少肝脏脂质蓄积。此外，APS 1通过调节Nrf-2/HO-1表达增加肝脏抗氧化活性。值得注意的是，APS 1操纵肠道微生物群，导致拟杆菌门/厚壁菌门的比例增加，并减少特定的NAFLD相关bacterias.Conclusion丰度：这些结果表明，APS 1通过调节脂质代谢和抗氧化活性，通过操纵特定的NAFLD相关的肠道微生物群在体内改善肝脏脂肪变性。
Scope: Non-alcoholic fatty liver disease (NAFLD) is a common disease that is concomitant with obesity, resulting in increased mortality. To date, the efficiency of NAFLD treatment still needs to be improved. Therefore, we aimed to evaluate the effect of Lactobacillus mali APS1, which was isolated from sugary kefir, on hepatic steatosis in rats fed a high-fat diet (HFD).Methods and results: Sprague Dawley rats were fed a control diet, a HFD with saline, and a HFD with APS1 intervention by gavage daily for 12 weeks. The results showed that APS1 significantly reduced body weight and body weight gain in HFD-fed rats. APS1 reduced hepatic lipid accumulation by regulating SIRT-1/PGC-1 alpha/SREBP-1 expression. Moreover, APS1 increased hepatic antioxidant activity by modulating Nrf-2/HO-1 expression. Notably, APS1 manipulated the gut microbiota, resulting in increasing proportions of the phylum Bacteroidetes/Firmicutes and reducing the abundance of specific NAFLD-associated bacteria.Conclusion: These results suggested that APS1 ameliorated hepatic steatosis by modulating lipid metabolism and antioxidant activity via manipulating specific NAFLD-associated gut microbiota in vivo.