An Upside Down View of Cholesterol's Condensing Effect: Does Surface Occupancy Play a Role?
An Upside Down View of Cholesterol's Condensing Effect: Does Surface Occupancy Play a Role?
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DOI：
10.1021/la100878s
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发表时间：
2010-04-20
期刊：
影响因子：
3.9
通讯作者：
Regen, Steven L.
中科院分区：
文献类型：
--
作者：
Janout, Vaclav;Turkyilmaz, Serhan;Regen, Steven L.
已将胆固醇的凝聚作用与羟基位于C-25位置（即25-OH'）的结构异构体的凝聚作用进行了比较，即在脂质膜中倾向于“倒置”取向的异构体。1,2-二肉豆醇- n-甘油-3-磷脂胆碱(DM PC)/胆固醇和DM PC/25-OH‘组成的混合单层膜的表面压力面积等温线表明，25-OH’的凝结作用弱于胆固醇。由1,2-二棕榈酰- san -甘油-3-磷脂胆碱（DPPC）制成的脂质体的最近邻识别测量也表明，25-OH'在生理相关的流体双分子层状态下具有较弱的凝聚作用。这些发现支持了表面占用在胆固醇的凝结作用中起作用。
The condensing action or cholesterol has been compared with that of a structural isomer having its hydroxyl group located at the C-25 position (i.e., 25-OH'), that is, an isomer favoring an "upside down" orientation in lipid membranes. Surface pressure area isotherms of mixed monolayers made from 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DM PC)/cholesterol and DM PC/25-OH' have established that 25-OH' has a weaker condensing effect than cholesterol. Nearest-neighbor recognition measurements in liposomes made from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) have also shown that 25-OH' has a weaker condensing effect in the physiologically relevant fluid bilayer state. These findings provide support for surface occupancy playing a role in the condensing action of cholesterol.