TRANSGENETIC STUDIES IMPLICATE INTERACTIONS BETWEEN HOMOLOGOUS PRP ISOFORMS IN SCRAPIE PRION REPLICATION
TRANSGENETIC STUDIES IMPLICATE INTERACTIONS BETWEEN HOMOLOGOUS PRP ISOFORMS IN SCRAPIE PRION REPLICATION
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DOI：
10.1016/0092-8674(90)90134-z
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发表时间：
1990-11-16
期刊：
影响因子：
64.5
通讯作者：
DEARMOND, SJ
中科院分区：
文献类型：
--
作者：
PRUSINER, SB;SCOTT, M;DEARMOND, SJ
用同时表达叙利亚仓鼠（Ha）和小鼠（Mo）朊蛋白（PrP）基因的转基因（Tg）小鼠来研究羊瘙痒症朊病毒复制的机制。四个Tg系表达HaPrP表现出不同的孵育时间范围从48到277天，这与HaPrP mRNA和HaPrPC呈负相关。Tg脑提取物的生物测定表明，朊病毒接种物决定了哪些朊病毒是从头合成的。用Ha朊病毒接种的Tg小鼠每克脑具有约109 ID 50单位的Ha朊病毒，
Transgenic (Tg) mice expressing both Syrian hamster (Ha) and mouse (Mo) prion protein (PrP) genes were used to probe the mechanisms of scrapie prion replication. Four Tg lines expressing HaPrP exhibited distinct incubation times ranging from 48 to 277 days, which correlated inversely with HaPrP mRNA and HaPrPC. Bioassays of Tg brain extracts showed that the prion inoculum dictates which prions are synthesized de novo. Tg mice inoculated with Ha prions had .apprx.109 ID50 units of Ha prions per gram of brain and