Effects of multiple factors on hyperhydricity of Allium sativum L
Effects of multiple factors on hyperhydricity of Allium sativum L
复制标题
多种因素对大蒜水分过高的影响
DOI：
10.1016/j.scienta.2017.02.010
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发表时间：
2017
影响因子：
4.3
通讯作者：
Wu Zhen
中科院分区：
文献类型：
--
作者：
Liu Min;Jiang Fangling;Kong Xiangyu;Tian Jie;Wu Zexiu;Wu Zhen
摘要高水分是植物试管培养过程中常见的一种生理障碍，严重影响植物的再生和快速繁殖。大蒜对水分过多很敏感。然而，多因素对大蒜高水分的影响尚不清楚。为了弄清大蒜高水分的发生规律，获得高效的再生体系，我们系统地研究了外植体、培养基组分、培养条件和外源添加剂对大蒜高水分的影响。结果表明，新梢比试管苗更容易脱水。花序诱导的芽比鳞茎诱导的芽更快，增殖系数也更高。基因型、生理年龄和外植体大小影响初代培养中芽的高含水率，而继代培养中影响植株的高含水率。较年轻的花序和较小的外植体更容易发生氢化。细胞分裂素和凝胶剂参与高水分的作用方式呈剂量依赖关系。高水分随着细胞分裂素浓度的增加而加重，并通过增加胶凝剂和蔗糖浓度、通风和光照强度来缓解。PH>6.0和<5.8的培养基易出现高渗水。试管苗和试管苗在MS培养基比在B5培养基中更容易发生氢化。50μM水杨酸、250μM抗坏血酸、10μM亚精胺和50μM过氧化氢均可缓解高血糖，但高浓度的过氧化氢和亚精胺可加重高血糖。甘露醇对高血糖无影响，而聚乙二醇6000可诱导高血糖。不同的细胞分裂素、胶凝剂和不同的外植体(包括基因型、器官类型、生理年龄和大小)处理下，芽的增殖和高水分含量呈正相关。在此基础上，建立了高增殖系数、低氢化率的大蒜再生体系。
Hyperhydricity is a common physiological disorder during plantin vitroculture and seriously affected regeneration and micropropagation of plants. Garlic is very susceptible to hyperhydricity. However, effects of multiple factors on hyperhydricity of garlic remain unclear. To clear the regularity of occurrence of hyperhydricity and to obtain a high-efficiency regeneration system of garlic, we systematically investigated effects of explants, media components, culture conditions, and exogenous additives on hyperhydricity. Our results showed that shoots were more easily hyperhydric than plantlets. Shoots induced by inflorescences showed a higher hyperhydric rate and proliferation coefficient than those induced by bulbs. Genotype, physiological age, and explant size affected hyperhydricity of shoots in initial culture, not that of plantlets in subculture. Younger inflorescence and smaller explant were more easily hyperhydric. Dose-dependent manners of cytokinins and gelling agents involved in hyperhydricity were found. Hyperhydricity was aggravated at increased cytokinin concentrations and was alleviated by increased gelling agent and sucrose concentrations, ventilation, and illumination intensity. Media with pH higher than 6.0 and lower than 5.8 resulted in more hyperhydricity. Shoots and plantlets were much more likely to be hyperhydric in MS medium than that in B5 medium. Hyperhydricity was relieved by 50 μM salicylic acid, 250 μM ascorbic acid, 10 μM spermidine, and 50 μM hydrogen peroxide, but aggravated by high concentrations of hydrogen peroxide and spermidine. Mannitol had no effect on hyperhydricity, whereas polyethylene glycol 6000 induced it. Positive correlations of shoot proliferation and hyperhydricity were found under different treatments of cytokinins, gelling agents, and explants which included genotype, organ type, physiology age and size. A regeneration system of garlic with high proliferation coefficient and low hyperhydric rate was established based on the results above.