The need for complex 3D culture models to unravel novel pathways and identify accurate biomarkers in breast cancer.
The need for complex 3D culture models to unravel novel pathways and identify accurate biomarkers in breast cancer.
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DOI：
10.1016/j.addr.2014.01.001
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发表时间：
2014-04
影响因子：
16.1
通讯作者：
Bissell, Mina J.
中科院分区：
文献类型：
--
作者：
Weigelt, Britta;Ghajar, Cyrus M.;Bissell, Mina J.
关键词：
最近对乳腺癌基因组畸变的分类揭示了该疾病在遗传水平上的多样性和复杂性。为了揭示乳腺癌中特定突变库和拷贝数变化对信号通路的功能影响，开发真正重现该疾病的模型系统至关重要。在这里，我们讨论了三维培养模型，目前正在使用或最近开发的正常乳腺上皮细胞和乳腺癌，包括原发性肿瘤和休眠的研究。我们讨论了从这些模型中获得的关于细胞信号传导和潜在的治疗策略的见解，以及需要满足的挑战，以产生适合高通量方法的异型乳腺癌模型系统。
The recent cataloging of the genomic aberrations in breast cancer has revealed the diversity and complexity of the disease at the genetic level. To unravel the functional consequences of specific repertoires of mutations and copy number changes on signaling pathways in breast cancer, it is crucial to develop model systems that truly recapitulate the disease. Here we discuss the three-dimensional culture models currently being used or recently developed for the study of normal mammary epithelial cells and breast cancer, including primary tumors and dormancy. We discuss the insights gained from these models in regards to cell signaling and potential therapeutic strategies, and the challenges that need to be met for the generation of heterotypic breast cancer model systems that are amenable for high-throughput approaches.
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影响因子：
24.3
作者：
Correia, Ana Luisa;Bissell, Mina J.
通讯作者：
Bissell, Mina J.
影响因子：
64.8
作者：
通讯作者：
--
层粘连蛋白和仿生细胞外弹性增强了乳腺上皮的功能分化。
影响因子：
11.4
作者：
Alcaraz, Jordi;Xu, Ren;Mori, Hidetoshi;Nelson, Celeste M.;Mroue, Rana;Spencer, Virginia A.;Brownfield, Doug;Radisky, Derek C.;Bustamante, Carlos;Bissell, Mina J.
通讯作者：
Bissell, Mina J.
影响因子：
4.8
作者：
Debnath, J;Muthuswamy, SK;Brugge, JS
通讯作者：
Brugge, JS
影响因子：
--
作者：
Costa Santos, Rosangela Portilho;Benvenuti, Ticiana Thomazine;Azevedo Koike Folgueira, Maria Aparecida
通讯作者：
Azevedo Koike Folgueira, Maria Aparecida