Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility.
Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility.
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表达人类免疫缺陷病毒易感性的 H9 细胞系的核型衍生。
DOI：
10.1093/jnci/84.24.1922
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Chen,TR
中科院分区：
文献类型：
--
作者：
Chen,TR
背景资料：已知T细胞淋巴瘤HUT 78细胞系和来源于HUT 78的CD 4阳性HT细胞系的H9克隆在等位酶（等位基因同工酶）模式和DNA指纹的基础上在遗传上是相同的，但是这些细胞系的染色体组成尚未确定。HT细胞系及其H9克隆携带对人类免疫缺陷病毒（HIV）的易感性。目的：本研究的目的是通过表征HUT 78和H9细胞系的核型，通过比较这些细胞系之间的染色体差异，并通过评估它们之间的关系，来检查与H9细胞特有的HIV易感性表达相关的特定染色体变化。制备风干的染色体片，用快速Giemsa-胰蛋白酶法描绘G-带。结果：两种细胞系的核型均较复杂，其中47个HUT 78中期分裂相和21个H9中期分裂相的染色体数目分布较复杂，G显带明显，核型分析结果表明，两种细胞系的典型核型均含有47%~ 65%的结构修饰标记染色体。HUT 78培养物的染色体数目分布较广。有两个不同的染色体众数在42和43和在73，这是由于存在三个活跃的生长亚系：两个亚二倍体（1 s）亚系（HUT 78/1 sA和HUT 78/1 sB）和一个超三倍体（2s）亚系（HUT 78/2s）。HUT 78/1 sA和HUT 78/1 sB之间存在5个相互的染色体置换，HUT 78/2在15个细胞中大部分染色体为双拷贝。H9和HUT 78/2shad有21个匹配标记，其中10个配对;含有21条拷贝数相同的染色体;始终缺乏8条同源正常染色体;染色体众数接近，分别为69和73。所有细胞系显然具有相同的祖细胞群，HUT 78/2s代表了HUT 78/15与H9的中间连接。染色体组学研究（如本文所述）可以提供数据（a）明确鉴定亚系的克隆细胞群，（B）检查同一祖细胞亚系之间的关系，以及（c）提供可能将细微的染色体变化与表型表达联系起来的线索。[J Natl Cancer Inst 84：1922-1926，1992]
Background: The T-cell lymphoma HUT78 cell line and the H9 clone of the CD4-positive HT cell line, derived from HUT78, are known to be genetically identical on the basis of allozyme (allelic isozyme) patterns and DNA fingerprinting, but the chromosome compositions of these cell lines have not been determined. The HT cell line and its H9 clone carry susceptibility to the human immunodeficiency virus (HIV).Purpose: The purpose of this study was to examine specific chromosome changes linking the expression of the HIV susceptibility that is unique to H9 cells by characterizing the karyo-types of the HUT78 and the H9 cell lines, by comparing chromosome differences between these lines, and by evaluating the relationships between them.Methods: Air-dried chromosome slides were prepared, and the fast Giemsa-trypsin method was used to delineate G-bands. The numerical distribution of chromosomes from the two cell lines was determined, and complete chromosome analysis in 47 HUT78 metaphases and 21 H9 metaphases with discernible G-banding was used to specify the modal karyotypes of each cell line.Results: Both cell lines had complex karyotypes that contained 47%–65% structurally modified marker chromosomes. The distribution of numbers of chromosomes in HUT78 cultures was broad. There were two distinct modal numbers of chromosomes at 42 and 43 and at 73, resulting from the presence of three actively growing sublines: two hypodiploid (1s) sublines (HUT78/1sA and HUT78/1sB) and a hypertriploid (2s) subline (HUT78/2s), respectively. HUT78/1sA and HUT78/1sB differed by five reciprocal chromosome replacements, and HUT78/2shad double copies of most of the chromosomes in 1scells. H9 and HUT78/2shad had 21 matching markers, 10 of which were paired; contained 21 chromosomes with the same number of copies; consistently lacked eight homologous normal chromosomes; and showed close modal numbers of chromosomes at 69 and 73, respectively.Conclusions: Derivation of the 2ssubline from the Is subline by poly-ploidization is apparent. All lines apparently had the same progenitor cell population, and HUT78/2srepresents an intermediate linking HUT78/15 to H9.Implications: These data should be useful for studying specific chromosome changes linking the expression of the HIV susceptibility unique to H9 cells. Karyologic studies such as those presented here can provide data (a) to clearly identify the clonal cell population of a subline, (b) to examine relationships among sublines of the same progenitor, and (c) to provide a clue that may link a subtle chromosome change to a phenotypic expression. [J Natl Cancer Inst 84:1922–1926, 1992]