Ten nucleotide cis element in the 3′-untranslated region of the GLUT1 glucose transporter mRNA increases gene expression via mRNA stabilization
Ten nucleotide cis element in the 3′-untranslated region of the GLUT1 glucose transporter mRNA increases gene expression via mRNA stabilization
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DOI：
10.1016/s0169-328x(98)00134-x
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发表时间：
1998-08-15
期刊：
影响因子：
--
通讯作者：
Pardridge, WM
中科院分区：
文献类型：
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作者：
Boado, RJ;Pardridge, WM
GLUT 1葡萄糖转运蛋白基因在转录后水平受到调控，位于GLUT 1 3 '-非翻译区（3'-UTR）nt 2181-2190的10个核苷酸（nt）顺式作用元件增加荧光素酶报告基因的瞬时表达。为了研究这种mRNA顺式元件的作用，在大鼠C6胶质瘤细胞中建立了表达荧光素酶报告基因的稳定转染子。GLUT 1 3 '-UTR的nt 2100-2300的插入导致报告基因mRNA和蛋白质的丰度与对照相比显著增加，与用放线菌素D测定的mRNA t（1/2）增加228%平行。在GLUT 1 3 '-UTR中缺失10 nt顺式作用元件将报告基因产物和mRNA t（1/2）的丰度降低至与对照克隆相似的水平。结果表明，牛GLUT 1 mRNA 3 '-UTR第2181-2190位核苷酸的顺式作用元件通过增强GLUT 1 mRNA的稳定性而增加GLUT 1基因的表达。(C)1998 Elsevier Science B. V.保留所有权利。
The GLUT1 glucose transporter gene is regulated at the post-transcriptional level, and a 10 nucleotide (nt) cis-acting element located at nt 2181-2190 of the GLUT1 3'-untranslated region (3'-UTR) increases the transient expression of a luciferase reporter gene. To investigate the role of this mRNA cis-element, stable transfectants expressing luciferase reporter genes were established in rat C6 glioma cells. Insertion of nt 2100-2300 of GLUT1 3'-UTR resulted in a marked increase in the abundance of both reporter gene mRNA and protein compared to the control, in parallel with a 228% increase in the mRNA t(1/2) determined with actinomycin D. Deletion of the 10 nt cis-acting element in the GLUT1 3'-UTR reduced the abundance of reporter gene products and the mRNA t(1/2) to levels similar to the control clone. Data suggest that the cis-acting element located at nt 2181-2190 of bovine GLUT1 mRNA 3'-UTR is responsible for increased GLUT1 gene expression via enhanced GLUT1 mRNA stabilization. (C) 1998 Elsevier Science B.V. All rights reserved.