Integrins contribute to the establishment and maintenance of cell polarity in the follicular epithelium of the Drosophila ovary
Integrins contribute to the establishment and maintenance of cell polarity in the follicular epithelium of the Drosophila ovary
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DOI：
10.1387/ijdb.072418af
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发表时间：
2008-01-01
影响因子：
0.7
通讯作者：
Martin-Bermudo, Maria D.
中科院分区：
文献类型：
--
作者：
Fernandez-Minan, Ana;Cobreros, Laura;Martin-Bermudo, Maria D.
上皮细胞极性的形成是发育过程中的一个关键过程。尽管细胞-细胞和细胞-细胞外基质(ECM)相互作用对细胞极性的诱导和定位已被证实，但ECM信号在发育中的上皮组织中控制细胞极性的分子机制仍不清楚。在这里，我们使用果蝇卵巢的卵泡上皮来研究整合素，主要的细胞-ECM受体，在建立顶基底部极性中所起的作用。成熟的滤泡细胞顶端面向胚线，基端与基底膜接触。我们的结果表明，整合素可能通过与基底膜的相互作用在卵泡细胞极化中发挥增强作用，因为只有在阻止与生殖系接触的情况下，它们才需要建立和/或维持卵泡细胞膜的不对称性。我们认为卵泡上皮极化的主要线索是与生殖细胞接触。此外，虽然干扰顶端和侧向极化信号会导致细胞凋亡，但我们在这里表明，整合素介导的抑制与基底膜的接触并不影响细胞存活。最后，我们提供的证据表明，整合素在体内定位上皮极性是必需的。
The generation of epithelial cell polarity is a key process during development. Although the induction and orientation of cell polarity by cell-cell and cell-extracellular matrix (ECM) interactions is well established, the molecular mechanisms by which signals from the ECM control cell polarity in developing epithelial tissues remain poorly understood. Here, we have used the follicular epithelium of the Drosophila ovary to investigate the role that integrins, the main cell-ECM receptors, play in the establishment of apicobasal polarity. Mature follicle cells have an apical side facing the germ line and a basal side in contact with a basement membrane. Our results show that integrins - presumably via interactions with the basement membrane - play a reinforcing role in follicle cell polarization, as they are required to establish and/or maintain follicle cell membrane asymmetry only when contact with the germ line is prevented. We suggest that the primary cue for polarization of the follicular epithelium is contact with the germline cells. In addition, while interfering with apical and lateral polarization cues leads to apoptosis, we show here that inhibition of contact with the basement membrane mediated by integrins does not affect cell survival. Finally, we provide evidence to suggest that integrins are required to orientate epithelial polarity in vivo.