Possible involvement of crosstalk cell-adhesion mechanism by endometrial CD26/dipeptidyl peptidase IV and embryonal fibronectin in human blastocyst implantation
Possible involvement of crosstalk cell-adhesion mechanism by endometrial CD26/dipeptidyl peptidase IV and embryonal fibronectin in human blastocyst implantation
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DOI：
10.1093/molehr/gal019
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发表时间：
2006-08-01
影响因子：
4
通讯作者：
Kikkawa, Fumitaka
中科院分区：
文献类型：
--
作者：
Shimomura, Yuji;Ando, Hisao;Kikkawa, Fumitaka
当人类囊胚通过透明带孵化，获得附着在子宫内膜上的能力时，胚胎和子宫之间的串扰可能代表了它们同步发育和分化的成功结果。CD26/二肽基肽酶IV被认为是着床期子宫内膜的标记分子。为了研究CD26在胚胎着床中的作用，我们将2细胞或4细胞期的冷冻剩余胚胎按计划生长的胚泡经酶处理后，制备了35个已孵化的囊胚。囊胚置于过表达cd26或模拟转染的对照单层细胞培养物上。cd26过表达显著提高了囊胚粘附率（53.3%比25.0%,P < 0.05），显著增大了滋养外胚层的生长面积（1.7倍，P < 0.05）。本研究的第二部分是利用相同的捐赠资源，在人类植入前胚胎中显示一种CD26配体——纤维连接蛋白的表达。单囊胚（2/2）和单早期囊胚（3/6）均检测到纤维连接蛋白mRNA，而单桑葚胚（0/5）未检测到纤维连接蛋白mRNA。间接免疫荧光技术证实了纤维连接蛋白在囊胚表面的定位。提示子宫内膜CD26与胚胎纤维连接蛋白的粘附机制可能参与了囊胚着床过程。
When human blastocysts hatch through the zona pellucida, gaining the ability to adhere to the endometrium, crosstalk between the embryo and the uterus may represent a successful outcome of their synchronized development and differentiation. CD26/dipeptidyl peptidase IV is known as a marker molecule of the implantation phase endometrium. To study the role of CD26 in implantation, 35 human hatched blastocysts were prepared by enzymatic treatment of expanded blastocysts that had been grown on schedule from frozen-thawed surplus embryos at the 2- or 4-cell stage. The blastocysts were placed on CD26-overexpressing or mock-transfected control monolayer cell cultures. The CD26-overexpression caused significantly higher blastocyst adhesion rate (53.3% versus 25.0%, P < 0.05) and significantly larger outgrowth area of trophectoderm (1.7-fold, P < 0.05). The second part of the present study was to show the expression of fibronectin, a CD26 ligand, in human preimplantation embryos, using the same donated resources. Fibronectin mRNA was detected by RT-PCR from the single hatched blastocyst (2/2) and from the single early blastocyst (3/6) but not from the single morula (0/5) samples. An indirect immunofluorescence technique verified the localization of fibronectin on the surface of the blastocyst. These results indicate that the adhesion mechanism by endometrial CD26 and embryonal fibronectin may be involved in human blastocyst implantation.