Regulation ofEthylene Biosynthesis inVirus-Infected Tobacco Leaves'
Regulation ofEthylene Biosynthesis inVirus-Infected Tobacco Leaves'
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病毒感染烟叶乙烯生物合成的调控
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
C. Vanloon
中科院分区：
文献类型：
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作者：
M. M. Delaatandleendert;C. Vanloon
在SamsunNN烟草对烟草花叶病毒(TMV)的超敏反应中，乙烯的产生被刺激了几倍。外源蛋氨酸ORS-腺苷脱氢酶(SAM)不增加健康或TMV侵染的叶盘的乙烯释放，尽管这两种前体都直接用于乙烯的产生。这表明，乙烯的产生没有控制在蛋氨酸浓度或可利用性的水平上，也没有控制在SAM产生或浓度的北部水平上。相反，1-氨基环丙烷-1-羧酸(ACC)能显著刺激乙烯的产生。因此，乙烯的生产主要限于AC生产的水平。通过测定蛋氨酸、SAM和ACC的浓度时程及其体内转化率，进一步研究了TMV过敏反应中乙烯产生的调节。内源蛋氨酸和蛋氨酸的浓度直到感染后期才受到影响。相反，在接近局部病变发展的时候，乙烯的产生先于AC的产生而显著增加。作为这种增加的结果，ACC在叶片组织中积累。只有在局部损伤明显后，将ACC转化为乙烯的能力才增加几倍，并伴随着AC含量的降低和乙烯产量的增加。烟草对TMV反应敏感的叶片中乙烯的产生是在AC的产生及其向乙烯的转化的水平上调节的。
Ethylene production wasstimulated severalfold during thehypersensitive reaction ofSamsunNN tobacco totobacco mosaic virus (TMV). Exogenous methionine orS-adenosyhnethlonlne (SAM)didnotincrease ethylene evolution fromhealthy orTMV-infected leaf discs, although both precursors weredirectiy available forethylene production. Thisindiates thatethylene production isnotcontrolled atthelevel ofmethionine concentration oravailability, noratthelevel ofSAM production or concentration. Incontrast, 1-aminocyclopropane-1-carboxylic acid (ACC) stimulated ethylene production considerably. Thus, ethylene production is primarily limited atthelevel ofACCproduction. Theregulation ofethylene production during thehypersensitive reaction toTMV wasfurther studied bydetermining thetimecourseofthe concentrations ofmethionine, SAM,andACC,aswell asthecourse of their invivo conversion rates. Endogenous concentrations ofmethionine andSAMremaned unaffected until late ininfection. Onthecontrary, the peakinethylene production nearthetime oflocal lesion development was preceded byalarge increase inACCproduction. Asa result ofthis increase, ACCaccumulated intheleaf tissue. Only after local lesions becamevisible, thecapacity toconvert ACC intoethylene increased severalfold, associated with asharp decrease inACCcontent andalarge increase inethylene production. Ethylene production intobacco leaves reacting hypersensitively toTMV isthusregulated atthelevel ofboththeproduction ofACCandits conversion toethylene.