A 36‐bp deletion in the alpha subunit of glutamate‐gated chloride channel contributes to abamectin resistance in Plutella xylostella
A 36‐bp deletion in the alpha subunit of glutamate‐gated chloride channel contributes to abamectin resistance in Plutella xylostella
复制标题
DOI：
10.1111/eea.12232
复制
发表时间：
2014-11
影响因子：
1.9
通讯作者：
Feng Liu;Xiuzhen Shi;Yanpo Liang;Qing-jun Wu;Bao-yun Xu;W. Xie;Shaoli Wang;Youjun Zhang;Nannan Liu
中科院分区：
文献类型：
--
作者：
Feng Liu;Xiuzhen Shi;Yanpo Liang;Qing-jun Wu;Bao-yun Xu;W. Xie;Shaoli Wang;Youjun Zhang;Nannan Liu
小菜蛾（DBM）、小菜蛾Plutella xylostella（L.）（鳞翅目：菜蛾科）是菜科作物如大白菜的主要害虫之一。它对阿维菌素的抗药性正在迅速发展，阿维菌素是中国和其他东南亚国家控制小菜蛾的主要杀虫剂。阿维菌素的靶标，谷氨酸门控氯离子通道（GluClα）的α亚基，被认为参与线虫和昆虫中阿维菌素抗性的发展。本研究调查了小菜蛾阿维菌素敏感和抗性菌株中GluClα的变体。PxGluClα序列的比较显示了三种变体，包括63 bp取代，36 bp缺失和65 bp插入。与敏感菌株相比，阿维菌素抗性菌株中36-bp缺失的频率要高得多，而63-bp取代和65-bp插入在抗性菌株和敏感菌株之间没有显着差异。PxGluClα（有或没有36 bp缺失）在非洲爪蟾（Daudin）卵母细胞中的体外表达表明，与野生型PxGluClα相比，36 bp缺失的PxGluClα对谷氨酸和阿维菌素的敏感性较低。这些发现表明，PxGluClα中的变体36-bp缺失可能在阿维菌素连续选择后赋予小菜蛾阿维菌素抗性，为该害虫的管理提供了新的见解，并有助于开发新的害虫防治试剂。
Diamondback moth (DBM), Plutella xylostella (L.) (Lepidoptera: Plutellidae), is one of the most destructive pests in Brassicaceae crops, such as Chinese cabbage (Brassica rapa L.). It is rapidly developing resistance to abamectin, the dominant insecticide utilized in controlling P. xylostella in China and other southeastern Asian countries. The target of abamectin, the alpha subunit of glutamate‐gated chloride channel (GluClα), is thought to be involved in the development of abamectin resistance in nematodes and insects. This study investigated variants of GluClα in both abamectin‐susceptible and resistant strains of P. xylostella. A comparison of the PxGluClα sequences revealed three variants, including a 63‐bp substitution, a 36‐bp deletion, and a 65‐bp insertion. The frequency of the 36‐bp deletion was much higher in the abamectin‐resistant strain compared to the susceptible strain, whereas the 63‐bp substitution and 65‐bp insertion showed no significant difference between the resistant and susceptible strains. The in vitro expression of PxGluClα (with or without the 36‐bp deletion) in Xenopus laevis (Daudin) oocytes indicated that PxGluClα with the 36‐bp deletion was less sensitive to both glutamate and abamectin compared to the wild‐type PxGluClα. These findings suggest that the variant 36‐bp deletion in PxGluClα may confer abamectin resistance in P. xylostella after continuous abamectin selection, providing new insights into the management of this pest and contributing to the development of new reagents for pest control.