Control of division patterns in explanted microspores ofTulipa gesneriana
Control of division patterns in explanted microspores ofTulipa gesneriana
复制标题
郁金香外植小孢子分裂模式的控制
作者:
I. Tanaka;M. Ito
SummaryIsolated microspores ofTulipa gesneriana, were cultured through the mitotic cycle. Microspore division was polar and formed typical pollen grains showing a generative-within-vegetative cell arrangement. The normal development of microspores was inhibited by colcemid, which produced frequently anomalous pollen with two nuclei similar in structure and staining properties. Colcemid seems to interfere with the nuclear differentiation by preventing the asymmetric division at the mitotic phase. After subsequent culture both anomalous and typical pollen developed a pollen tube.