Produced β-hydroxybutyrate after β-hydroxy-β-methylbutyrate (HMB) administration may contribute HMB function in mice.
Produced β-hydroxybutyrate after β-hydroxy-β-methylbutyrate (HMB) administration may contribute HMB function in mice.
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β-羟基-β-甲基丁酸酯（HMB）给药后产生的β-羟基丁酸酯可能有助于小鼠的HMB功能。
DOI：
10.1016/j.bbrep.2021.101097
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发表时间：
2021-09
影响因子：
2.7
通讯作者：
Furuse M
中科院分区：
文献类型：
--
作者：
Ikeda K;Takahashi M;Aburaya S;Harada D;Ikeda M;Kitagawa Y;Soma Y;Izumi Y;Bamba T;Furuse M
β-羟基-β-甲基丁酸（HMB）是支链氨基酸亮氨酸代谢的中间体。HMB对骨骼肌功能有几种已证实的影响，其中一些是矛盾的。此外，外源性HMB摄入对中间代谢物水平的影响尚不清楚。因此，我们研究了小鼠口服HMB后HMB代谢物的变化。首先，用蒸馏水或HMB（0.215 g/10 mL/kg）处理ICR小鼠。在给药后0、1、6、12和24 h进行采样。然后，ICR小鼠给予蒸馏水或HMB（0.215 g/10 mL/kg/d）10 d。给予HMB的小鼠在HMB给药后1小时显示肝脏β-甲基巴豆酰-CoA显著增加，血浆和腓肠肌中β-羟基丁酸盐增加。给药HMB 10 d的小鼠表现出显著的摄食量和体重下降;然而，腓肠肌的相对重量显著增加。这些结果可能归因于外源性HMB导致的β-羟基丁酸酯增加，因为β-羟基丁酸酯抑制摄食并抑制骨骼肌钙蛋白酶。结论：HMB代谢产物β-羟基丁酸在HMB的功能中起重要作用。HMB可提高血浆和腓肠肌中β-羟基丁酸的水平。组织和血浆中的HMB增加在6小时内恢复至原始水平。HMB处理10 d可抑制摄食量和降低体重。尽管HMB处理的小鼠体重下降，但腓肠肌重量增加。
β-Hydroxy-β-methylbutyrate (HMB) is an intermediate in the metabolism of the branched-chain amino acid leucine. HMB has several demonstrated effects on skeletal muscle function, some of which are contradictory. In addition, the effect of exogenous HMB intake on the levels of intermediate metabolites is not known. Therefore, we investigated changes in HMB metabolites after oral HMB administration in mice. First, ICR mice were treated with either distilled water or HMB (0.215 g/10 mL/kg). Sampling was performed at 0, 1, 6, 12, and 24 h after administration. Next, ICR mice were given distilled water or HMB (0.215 g/10 mL/kg/d) for 10 d. Mice given HMB shown a significant increase in liver β-methylcrotonyl-CoA and increased β-hydroxybutyrate in plasma and the gastrocnemius muscle 1 h after HMB administration. Mice administered HMB for 10 d showed significantly decreased food intake and body weight; however, the relative weight of the gastrocnemius muscle was significantly increased. These results may be attributed to an increase in β-hydroxybutyrate resulting from exogenous HMB, since β-hydroxybutyrate inhibits food intake and suppresses skeletal muscle catabolism. In conclusion, β-hydroxybutyrate, a metabolite of HMB, was found to play an important role in the function of HMB. β-Hydroxybutyrate levels in plasma and gastrocnemius were enhanced by HMB. HMB increases in tissues and plasma returned to original levels within 6 h. HMB treatment for 10 d suppressed food intake and decreased body weight. Gastrocnemius weight was increased despite deceased body weight in HMB-treated mice.
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影响因子：
29
作者：
Wagner GR;Bhatt DP;O'Connell TM;Thompson JW;Dubois LG;Backos DS;Yang H;Mitchell GA;Ilkayeva OR;Stevens RD;Grimsrud PA;Hirschey MD
通讯作者：
Hirschey MD
影响因子：
6.1
作者：
Fushimi, Tatsuya;Izumi, Yoshihiro;Bamba, Takeshi
通讯作者：
Bamba, Takeshi
乙酰化促进 BCAT2 降解，抑制 BCAA 分解代谢和胰腺癌生长
影响因子：
39.3
作者：
Lei, Ming-Zhu;Li, Xu-Xu;Lei, Qun-Ying
通讯作者：
Lei, Qun-Ying
影响因子：
3.3
作者：
Nissen, S;Sharp, R;Abumrad, N
通讯作者：
Abumrad, N
影响因子：
11.2
作者：
Smith, HJ;Wyke, SM;Tisdale, MJ
通讯作者：
Tisdale, MJ