REDISTRIBUTION OF ACTIN AND FASCIN IN SEA-URCHIN EGGS AFTER FERTILIZATION
REDISTRIBUTION OF ACTIN AND FASCIN IN SEA-URCHIN EGGS AFTER FERTILIZATION
复制标题
DOI：
10.1002/cm.970010104
复制
发表时间：
1980-01-01
影响因子：
--
通讯作者：
BRYAN, J
中科院分区：
文献类型：
--
作者：
OTTO, JJ;KANE, RE;BRYAN, J
受精后，海胆卵皮质发生结构变化，包括肌动蛋白丝组装和组织成微绒毛。抗筋膜蛋白定位这种肌动蛋白交联蛋白在受精卵皮层的微绒毛，但没有组织染色是存在于未受精的皮层。使用DNA酶I抑制测定法测定蛋的肌动蛋白含量表明肌动蛋白是约占总蛋白质的1.4%。大约90%的肌动蛋白可溶于未受精卵的低钙等渗提取物中，而只有60-65%可以在受精卵的相同提取物中回收。使用放射免疫测定法对肌成束蛋白的类似测量表明该分子代表apprx。总卵蛋白的0.3%，基本上所有这些都在未受精卵的低钙等渗提取物中回收。受精后，只有65-70%的这种肌动蛋白交联蛋白处于可溶相。这些结果表明，受精后的肌动蛋白和肌成束蛋白的不同的溶解度，当间接免疫荧光染色定位肌成束蛋白在微绒毛，并与肌成束蛋白组织新聚合的肌动蛋白丝到微绒毛核心的想法是一致的。肌动蛋白和肌成束蛋白的量纳入受精后的皮质和卵表面上的微绒毛的数量表明，测量值是足以占微绒毛伸长。
Following fertilization, the sea urchin egg cortex undergoes a structural change involving the assembly and organization of actin filaments into microvilli. Antifascin localizes this actin cross-linking protein in the microvilli of the fertilized egg cortex but no organized staining is present in the unfertilized cortex. Determination of the actin content of eggs using the DNAse I inhibition assay indicates that actin is .apprx. 1.4% of the total protein. Approximately 90% of this actin is soluble in low Ca isotonic extracts of unfertilized eggs while only 60-65% can be recovered in identical extracts of fertilized eggs. Similar measurements for fascin using a radioimmunoassay indicate this molecule represents .apprx. 0.3% of the total egg protein, essentially all of which is recovered in low Ca isotonic extracts of unfertilized eggs. After fertilization only 65-70% of this actin cross-linking protein is in the soluble phase. These results demonstrate a different solubility for actin and fascin after fertilization, when the indirect immunofluorescence staining localizes fascin in the microvilli, and are consistent with the idea that fascin organizes newly polymerized actin filaments into the microvillar cores. The amounts of actin and fascin incorporated into the cortex after fertilization and the number of microvilli on the egg surface indicate that the measured values are sufficient to account for microvillar elongation.