Bacteriophage Mu sites and functions involved in the inhibition of lambda::mini-Mu growth.
Bacteriophage Mu sites and functions involved in the inhibition of lambda::mini-Mu growth.
复制标题
噬菌体 Mu 位点和功能参与抑制 lambda::mini-Mu 生长。
DOI：
10.1016/0042-6822(90)90463-2
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发表时间：
1990
期刊：
影响因子：
3.7
通讯作者：
Howe,MM
中科院分区：
文献类型：
--
作者：
Glasgow,AC;Miller,JL;Howe,MM
为了更好地了解导致抑制λ：：Mini-Mu生长的Mini-Mu定向过程的本质，我们对λ：：Mini-Mu噬菌体的自发缺失突变体进行了鉴定。在分析缺失终点、Mini-Mu复制功能以及整合和抑制特性的基础上，将λ：：mini-Mu缺失突变体分为五类，分别定义了参与λ：：mini-Mu生长抑制的Mu位点和功能。1类突变体仍然表现出λ：：mini-Mu生长抑制，它们共同删除了mini-Mu编码的所有Mu晚期功能。2类和5类突变体在抑制和整合方面表现出显性缺陷，分别删除了右侧和左侧的mini-Mu附着位点。分别缺失MuBorA和B基因的3类和4类噬菌体在生长抑制方面表现出隐性缺陷。这些突变体的性质决定了Mu复制功能A和B以及Mu连接位点对于抑制λ：：Mini-Mu生长是必不可少的。观察到Mu复制所必需的位点和功能在抑制λ：：Mini-Mu生长中也具有必要的作用，这表明抑制是由于Mini-Mu促进λ：：Mini-Mu发育的复制干扰所致。
To better understand the nature of the mini-Mu-directed process which results in inhibition of λ:: mini-Mu growth we characterized spontaneous deletion mutants of the λ:: mini-Mu phage. On the basis of analysis of the deletion endpoints, mini-Mu replication functions, and integration and inhibition properties, the λ:: mini-Mu deletion mutants were divided into five classes which define the Mu sites and functions involved in λ::mini-Mu growth inhibition. Class 1 mutants, which still exhibit λ::mini-Mu growth inhibition, collectively delete all the Mu late functions encoded by the mini-Mu. Class 2 and 5 mutants, which showcis-dominant defects in inhibition and integration, delete the right and left mini-Mu attachment sites, respectively. Phages of Classes 3 and 4, which delete the MuBorAandBgenes, respectively, show recessive defects in growth inhibition. The properties of these mutants define the Mu replication functions, A and B, and the Mu attachment sites as essential for the inhibition of λ:: mini-Mu growth. The observation that the sites and functions essential for Mu replication also have requisite roles in the inhibition of λ:: mini-Mu growth suggests that inhibition results from mini-Mu-promoted replicative interference of λ::mini-Mu development.