Adolescent rats exposed to repeated ethanol treatment show lingering behavioral impairments
Adolescent rats exposed to repeated ethanol treatment show lingering behavioral impairments
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DOI：
10.1097/01.alc.0000175012.77756.d9
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发表时间：
2005-08-01
影响因子：
3.2
通讯作者：
Sircar, D
中科院分区：
文献类型：
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作者：
Sircar, R;Sircar, D
背景：反复乙醇处理对青少年和成年大鼠的水迷宫表现有不同的影响。本研究进行了确定乙醇诱导的赤字在水迷宫performance.Methods：青春期和成年雄性大鼠进行反复乙醇或生理盐水治疗的年龄特异性逆转。实验大鼠每天注射2 g/kg乙醇（腹膜内），连续5天（第1-5天），并在乙醇处理后30分钟在Morris水迷宫的隐藏平台任务中进行测试;对照大鼠接受等容盐水。在训练的最后一天，所有大鼠在探测试验和线索视觉任务进行了测试。经过4-25天的无乙醇期，大鼠在水迷宫.Results重新测试：青春期乙醇处理的大鼠有显着较高的lavelance和游泳更远的距离找到隐藏的平台，相比，年龄匹配的盐水对照组大鼠。乙醇大鼠也表现出增加的拥抱时间，即，与对照组大鼠相比，在水池周围花费的时间明显更多。在探测试验中，与青少年盐水大鼠相比，乙醇大鼠在目标象限花费的时间更少。然而，乙醇和盐水处理的大鼠在游泳速度或视觉任务表现方面没有差异。在最后一次乙醇/生理盐水处理后4天（第9天）、7天（第12天）和25天（第30天），在水迷宫中对实验和对照大鼠进行重新测试;在这些天未进行注射。在所有复检日，乙醇处理的大鼠继续表现不佳。成年雄性大鼠的乙醇治疗急剧增加了潜伏期和距离，以找到隐藏的平台，但与青春期酒精大鼠不同，它们在探测试验中的表现与成年盐水大鼠没有差异。此外，游泳速度和视觉任务的表现，成年大鼠的乙醇暴露显着影响。在重新测试，他们的表现并没有从成年对照rates.Conclusions不同：暴露于乙醇的青少年大鼠表现出赤字的水迷宫性能，增加了拥抱时间，并未能赶上对照组大鼠在乙醇治疗期后的几周内结束。成年酒精大鼠表现出一些行为功能障碍（寻找隐藏平台的潜伏期和距离增加），但游泳有问题，在探测试验中，它们的表现与对照大鼠一样。此外，在成年大鼠中，乙醇诱导的损伤在乙醇治疗结束后迅速逆转，这一发现表明运动协调受损比真正的学习缺陷更严重。总之，这些数据表明，反复乙醇处理青少年大鼠，但不是成年大鼠，表现出长期的迷宫性能受损。
Background: Repeated ethanol treatment has been reported to differentially affect water maze performance in adolescent and adult rats. The present study was undertaken to determine the age-specific reversal of ethanol-induced deficit in water maze performance.Methods: Adolescent and adult male rats were subjected to repeated ethanol or saline treatments. Experimental rats were injected daily with 2 g/kg ethanol (intraperitoneally) for five consecutive days (Days 1-5) and tested in the hidden platform task of the Morris water maze 30 minutes after ethanol treatment; control rats received isovolumetric saline. On the last training day, all rats were tested in the probe trial and in the cued visual task. After an ethanol-free period of 4-25 days, rats were retested in the water maze.Results: Adolescent ethanol-treated rats had significantly higher latencies and swam greater distances to find the hidden platform, compared to age-matched saline control rats. Ethanol rats also showed increased hug time, i.e., spent significantly more time near the periphery of the pool than control rats. In the probe trial, compared to adolescent saline rats, ethanol rats spent less time in the target quadrant. However, there was no difference between ethanol- and saline-treated rats in the swim speed or in the visual task performance. Experimental and control rats were retested in the water maze 4 days (Day 9), 7 days (Day 12), and 25 days (Day 30) after the last ethanol/saline treatment; no injections were given on those days. Ethanol-treated rats continued to do poorly on all retest days. Ethanol treatment in adult male rats acutely increased latency and distance to find the hidden platform, but unlike adolescent alcohol rats, their performance in the probe trial did not differ from adult saline rats. Also, swim speed and visual task performance of adult rats were significantly affected by ethanol exposure. During retesting, their performance did not differ from adult control rats.Conclusions: Adolescent rats exposed to ethanol showed deficits in water maze performance, had increased hug time, and failed to catch up with control rats during the weeks after the ethanol treatment period was over. Adult alcohol rats showed some behavioral dysfunction (increased latency and distance to find the hidden platform) but had problems swimming, and in the probe trial they performed as well as control rats. Also, in adult rats, ethanol-induced impairments were quickly reversed after the ethanol treatment was over, a finding that suggests impaired motor coordination more than a true learning deficit. Together, these data indicate that repeated ethanol treatment in adolescent rats, but not adult rats, show long-term impairments in maze performance.