Chondroitin sulfate demarcates astrocytic territories in the mammalian cerebral cortex
Chondroitin sulfate demarcates astrocytic territories in the mammalian cerebral cortex
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DOI：
10.1016/j.neulet.2010.07.064
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发表时间：
2010-10-08
影响因子：
2.5
通讯作者：
Wanaka, Akio
中科院分区：
文献类型：
--
作者：
Horii-Hayashi, Noriko;Tatsumi, Kouko;Wanaka, Akio
哺乳动物大脑皮层灰质中相邻的星形胶质细胞以瓷砖状的方式组织，彼此分离，形成称为“非重叠区域”的离散区域。我们之前报道了一种抗硫酸软骨素（CS）抗体CS-56，标记了皮层星形胶质细胞亚群，我们根据其形态特征将其命名为蒲公英钟状结构（DACS）。在本研究中，我们发现另一种抗cs抗体（anti-CS- c）也能检测到DACS，形态学分析显示单个DACS平均包裹5 - 6个神经元体，这表明DACS与单个星形胶质细胞区域重合。CS-C和胶质原纤维酸性蛋白（glial fibrillary acid protein， GFAP）的双标记在成年小鼠大脑皮层的两个区域有轻微的重叠。3周龄和7周龄小鼠单个DACS包裹的神经元数量没有变化，而7周龄小鼠的DACS过程比3周龄小鼠更广泛。此外，在3周龄和7周龄小鼠中，单个DACS区域的测量值显着增加了45%。此外，在人类、猴子和家猪的大脑中均发现了DACS，而在野鸭的大脑中未发现DACS，这表明DACS在哺乳动物物种中是保守的。综上所述，CS划分了一定数量的皮质星形胶质细胞的区域，大脑皮层由富含CS的星形胶质细胞和缺乏CS的星形胶质细胞以马赛克的方式组成。2010爱思唯尔爱尔兰有限公司版权所有。
Adjacent astrocytes in the grey matter of the mammalian cerebral cortex are organized in a tile-like manner and separated from one another, forming discrete domains named "non-overlapping territories". We have previously reported that an anti-chondroitin sulfate (CS) antibody, CS-56, marks a subpopulation of cortical astrocytes which we named the dandelion clock-like structure (DACS) based on its morphological characteristics. In the present study, we found that another anti-CS antibody (anti-CS-C) was also able to detect the DACS and the morphological analysis revealed that a single DACS enwrapped five to six neuronal somata on average, which indicated that DACS coincided with a single astrocyte territory. Double labeling of CS-C and glial fibrillary acidic protein (GFAP) showed a slight overlap between the two territories in the adult cerebral cortex of mice. The neuron number enwrapped by a single DACS was unchanged between 3- and 7-week-old mice, while more extensive processes of DACSs were found in 7-week-old mice compared with those in 3-week-old ones. Moreover, the measurement of a single DACS area was significantly increased by 45% between 3- and 7-week-old mice. In addition, DACSs were found in human, monkey, and domestic pig brains, but not mallard ones, indicating that DACS was conserved in mammalian species. Taken together, CS demarcates territories of a certain population of cortical astrocytes and the cerebral cortex is composed of CS-rich astrocytes and -poor astrocytes in a mosaic fashion. (C) 2010 Elsevier Ireland Ltd. All rights reserved.