PROPERTIES OF VOLTAGE-GATED CURRENTS OF MICROGLIA DEVELOPED USING MACROPHAGE-COLONY-STIMULATING FACTOR
PROPERTIES OF VOLTAGE-GATED CURRENTS OF MICROGLIA DEVELOPED USING MACROPHAGE-COLONY-STIMULATING FACTOR
复制标题
DOI：
10.1007/bf00373889
复制
发表时间：
1995-08-01
影响因子：
4.5
通讯作者：
HEINEMANN, U
中科院分区：
文献类型：
--
作者：
EDER, C;FISCHER, HG;HEINEMANN, U
从小鼠新生脑细胞培养中分离出小胶质细胞，其中巨噬细胞/小胶质细胞生长因子巨噬细胞集落刺激因子（M-CSF）的补充刺激了它们的发育。利用膜片钳技术的全细胞结构，从这些小胶质细胞中记录电压门控膜电流。超极化诱导向内整流K+电流，如未处理培养的小胶质细胞所述。当细胞外NaCl被n -甲基-d -氨基葡萄糖胺（NMG）取代时，这种失活被消除，从而表明Na+部分阻断了K+电导。向内的整流电流也被细胞外施加的Cs+或Ba2+阻断。它们在细胞外四乙基氯化铵（TEA）治疗后略有减少，但4-氨基吡啶（4-AP）不受影响。在长时间的去极化电压脉冲作用下，电池表现出缓慢激活的H+电流，该电流可以通过无机多价阳离子（Ba2+, Cd2+, Co2+, La3+ Ni2+, Zn2+）以及4-AP或TEA来降低。基于它们的动力学和药理学特征，在m - csf培养的小胶质细胞上检测到的两种电流都对应于巨噬细胞的内向整流和H+电流。
Microglia were isolated from a murine neonatal brain cell culture in which their development had been stimulated by supplementation with the macrophage/microglial growth factor macrophage colony-stimulating factor (M-CSF). Using the whole-cell configuration of the patch-clamp technique, voltage-gated membrane currents were recorded from these microglial cells. Hyperpolarization induced inward rectifying K+ currents, as described for microglia from untreated cultures. These currents activated negative to the K+ equilibrium potential and, with a strong hyperpolarization, displayed time-dependent inactivation, The inactivation was abolished when extracellular NaCl was replaced by N-methyl-D-glucamine (NMG), thereby indicating a partial block of this K+ conductance by Na+. Inward rectifying currents were also blocked by extracellularly applied Cs+ or Ba2+. They were slightly diminished following treatment with extracellular tetraethylammonium chloride (TEA) but were not affected by 4-aminopyridine (4-AP). Upon long lasting depolarizing voltage pulses to potentials positive to O mV, the cells exhibited a slowly activating H+ current which could be reduced by application of inorganic polyvalent cations (Ba2+, Cd2+, Co2+, La3+ Ni2+, Zn2+) as well as by 4-AP or TEA. Based on their kinetics and pharmacological characteristics, both currents detected on M-CSF-grown microglia are suggested to correspond to the inward rectifier and the H+ current of macrophages.