High-fat diet up-regulates caveolin-1 expression in aorta of diet-induced obese but not in diet-resistant rats
High-fat diet up-regulates caveolin-1 expression in aorta of diet-induced obese but not in diet-resistant rats
复制标题
高脂肪饮食上调饮食诱导的肥胖大鼠主动脉中的caveolin-1表达，但在饮食抵抗大鼠中则不然
DOI：
10.1016/j.cardiores.2007.05.028
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发表时间：
2007-10-01
影响因子：
10.8
通讯作者：
Sun, Xiufa
中科院分区：
文献类型：
--
作者：
Yang, Nianhong;Ying, Chenjiang;Sun, Xiufa
目的：Caveolin-1（Cav-1）被认为是内皮型一氧化氮合酶（eNOS）的负性调节因子，影响多种心血管功能。本研究的目的是探讨高脂饮食对血管Cav-1表达和eNOS活性的影响。方法：雄性远交系Sprague-Dawley大鼠喂饲高脂饮食15周，诱导肥胖。然后将饮食诱导的肥胖（DIO）大鼠分为两组。一组（DIO-LF）喂以正常饮食，另一组（DIO-HF）沿着饮食抵抗（DR）大鼠喂以高脂饮食8周以上。Cav-1的表达采用逆转录（RT）-PCR，蛋白质印迹和免疫组织化学技术。用Griess试剂检测NOS活性。结果：高脂饮食可上调DID大鼠主动脉Cav-1的表达，下调eNOS的表达，但对DR大鼠主动脉Cav-1的表达无明显影响。在切换到低脂饮食8周后，这些影响在DID大鼠中被逆转。DIO大鼠主动脉NOS活性降低，但DR大鼠主动脉NOS活性未降低。DIO-HF和DIO-LF组DID大鼠血管PKB/Akt和eNOS（Ser 1177）的磷酸化水平均明显升高。结论：高脂饮食导致大鼠血管NOS活性降低，Cav-1的表达上调是其部分原因。(C)2007年欧洲心脏病学会。Elsevier B. V.出版，保留所有权利。
Objective: Caveolin-1 (Cav-1) is considered as a negative regulator of endothelial nitric oxide synthase (eNOS) and influences various cardiovascular functions. The objective of the study is to investigate the effects of high-fat diet on vascular Cav-1 expression and eNOS activities.Methods: Male outbred Sprague-Dawley rats were fed with a high-fat diet for 15 weeks to induce obesity. The diet-induced obese (DIO) rats were then divided into two groups. One group (DIO-LF) was fed a normal diet and another group (DIO-HF) along with diet-resistance (DR) rats were fed a high-fat diet for 8 more weeks. Cav-1 expression was determined using reverse transcription (RT)-PCR, Western blotting, and immunohistochemistry techniques. NOS activities were assessed using Griess reagents. Protein kinase B (PKB/Akt) and eNOS expression and phosphorylation were determined by Western blotting.Results: A high-fat diet up-regulated Cav-1 and down-regulated eNOS expression in aorta of DID rats, but not in that of DR rats. These effects were reversed in DID rats after switching to a low-fat diet for 8 weeks. Decreased NOS activities in aortas were observed in DIO rats, but not in DR rats. Phosphorylation of PKB/Akt and eNOS (Ser1177) were enhanced in aortas of DID rats of both DIO-HF and DIO-LF groups.Conclusion: These findings suggest that the decrease of vascular NOS activities in rats fed a high-fat diet were due, at least in part, to the upregulation of Cav-1 expression. (C) 2007 European Society of Cardiology. Published by Elsevier B.V. All rights reserved.